A colorimetric plasma kallikrein substrate
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D-Pro-Phe-Arg-pNA (hydrochloride)

Item No. 27569

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
D-prolyl-L-phenylalanyl-N-(4-nitrophenyl)-L-argininamide, dihydrochloride
CAS Number
62354-56-7
Synonyms
  • pFR-pNA
  • Plasma Kallikrein Chromogenic Substrate
  • D-Pro-Phe-Arg-p-nitroaniline
Molecular Formula
C26H34N8O5 • 2HCl
Formula Weight
611.5
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
312 nm
SMILES
O=C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CCCNC(N)=N)C(NC2=CC=C([N+]([O-])=O)C=C2)=O)=O)[C@@H]3NCCC3.Cl.Cl
InChi Code
InChI=1S/C26H34N8O5.2ClH/c27-26(28)30-15-5-9-21(24(36)31-18-10-12-19(13-11-18)34(38)39)32-25(37)22(16-17-6-2-1-3-7-17)33-23(35)20-8-4-14-29-20;;/h1-3,6-7,10-13,20-22,29H,4-5,8-9,14-16H2,(H,31,36)(H,32,37)(H,33,35)(H4,27,28,30);2*1H/t20-,21+,22+;;/m1../s1
InChi Key
CFOCUZJDBWPWPX-XZQQPLIMSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $90

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    50 mg

    $168

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    100 mg

    $315

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    250 mg

    $696

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    Product Description

    D-Pro-Phe-Arg-pNA is a colorimetric substrate for plasma kallikrein.1 Plasma kallikrein preferentially binds to and cleaves the D-Pro-Phe-Arg (PFR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of plasma kallikrein activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tans, G., Rosing, J., Berrettini, M., et al. Autoactivation of human plasma prekallikrein. The Journal of Biological Chemisty 262(23), 11308-11314 (1987).