A fluorescent chloride indicator
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SPQ

Item No. 27604

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6-methoxy-1-(3-sulfopropyl)-quinolinium, inner salt
CAS Number
83907-40-8
Molecular Formula
C13H15NO4S
Formula Weight
281.3
Purity
≥95%
Emission
445 nm
Excitation
320 and 350 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: soluble
SMILES
COC1=CC(C=CC=[N+]2CCCS([O-])(=O)=O)=C2C=C1
InChi Code
InChI=1S/C13H15NO4S/c1-18-12-5-6-13-11(10-12)4-2-7-14(13)8-3-9-19(15,16)17/h2,4-7,10H,3,8-9H2,1H3
InChi Key
ZVDGOJFPFMINBM-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    100 mg

    $94

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    250 mg

    $218

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    500 mg

    $412

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    Product Description

    SPQ is a fluorescent chloride indicator.1 Its fluorescence is rapidly quenched by chloride in vitro. It has been used to assess voltage-sensitive chloride transport in placental microvillus vesicles. It has also been used to continuously quantify intracellular calcium efflux induced by parathyroid hormone, forskolin (Item No. 11018), and phorbol 12-myristate 13-acetate (PMA; Item No. 10008014) in isolated human erythrocytes.2 In addition, SPQ has been used to determine the type of ion conductance induced by the cystic fibrosis transmembrane conductance regulator (CFTR) in HEK293 cells expressing pTrial10-CRTR2.3 SPQ displays excitation maxima of 320 and 350 nm and an emission maximum of 445 nm.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Illsley, N.P., and Verkman, A.S. Membrane chloride transport measured using a chloride-sensitive fluorescent probe. Biochemistry 26(5), 1215-1219 (1987).

    2. Soldati, L., Adamo, D., Spaventa, R., et al. Chloride fluxes activated by parathyroid hormone in human erythrocytes. Biochem. Biophys. Res. Commun. 269(2), 470-473 (2000).

    3. MacVinish, L.J., Gill, D.R., Hyde, S.C., et al. Chloride secretion in the trachea of null cystic fibrosis mice: the effects of transfection with pTrial10-CFTR2. J. Physiol. 499(Pt. 3), 677-687 (1997).