A colorimetric serine protease substrate
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MeO-Suc-RPY-pNA (trifluoroacetate salt)

Item No. 27706

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N2-(4-methoxy-1,4-dioxobutyl)-L-arginyl-L-prolyl-N-(4-nitrophenyl)-L-tyrosinamide, trifluoroacetate salt
Synonyms
  • Methoxy-Succinyl-Arg-Pro-Tyr-pNA
  • Methoxy-Succinyl-Arg-Pro-Tyr-p-nitroanilide
  • Serine Protease Chromogenic Substrate
  • S 2586
Molecular Formula
C31H40N8O9 • XCF3COOH
Formula Weight
668.7
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 20mg/mLDMSO: 3mg/mLPBS (pH 7.2): 3mg/mL
λmax
225, 316 nm
SMILES
COC(CCC(N[C@@H](CCCNC(N)=N)C(N1CCC[C@H]1C(N[C@@H](CC2=CC=C(O)C=C2)C(NC3=CC=C([N+]([O-])=O)C=C3)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C31H40N8O9.C2HF3O2/c1-48-27(42)15-14-26(41)36-23(4-2-16-34-31(32)33)30(45)38-17-3-5-25(38)29(44)37-24(18-19-6-12-22(40)13-7-19)28(43)35-20-8-10-21(11-9-20)39(46)47;3-2(4,5)1(6)7/h6-13,23-25,40H,2-5,14-18H2,1H3,(H,35,43)(H,36,41)(H,37,44)(H4,32,33,34);(H,6,7)/t23-,24-,25-;/m0./s1
InChi Key
OSKBXHZCWLGBBY-NAGNLMCHSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $36

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    5 mg

    $153

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    10 mg

    $268

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    25 mg

    $587

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    Product Description

    MeO-Suc-RPY-pNA is a colorimetric substrate for serine proteases, including trypsin, chymotrypsin, kallikrein-related peptidase 7 (KLK7), and kallikrein-related peptidase 3/prostate-specific antigen.1,2,3 Serine proteases preferentially bind to and cleave the Arg-Pro-Tyr (RPY) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of serine protease activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Saedi, M.S., Mikolajczyk, S.D., Grauer, L., et al. Methods to detect HK2 polypeptides. EP 0 974 057 B1, (2005).

    2. de Veer, S.J., Furio, L., Swedberg, J.E., et al. Selective substrates and inhibitors for kallikrein-related peptidase 7 (KLK7) shed light on KLK proteolytic activity in the stratum corneum. J. Invest. Dermatol. 137(2), 430-439 (2017).

    3. Clark, M.R., Aliyar, H.A., Lee, C.W., et al. Enzymatic triggered release of an HIV-1 entry inhibitor from prostate specific antigen degradable microparticles. Int. J. Pharm. 413(1-2), 10-18 (2011).