A fluorogenic substrate for YFV NS3 and DV NS2B/3 serine proteases
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Bz-Nle-KRR-AMC (hydrochloride)

Item No. 27710

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-benzoyl-L-norleucyl-L-lysyl-L-arginyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-argininamide, hydrochloride
CAS Number
863975-32-0
Synonyms
  • Benzoyl-Nle-Lys-Arg-Arg-AMC
  • Benzoyl-Nle-Lys-Arg-Arg-7-amino-4-methylcoumarin
  • Bz-Nle-Lys-Arg-Arg-AMC
Molecular Formula
C41H60N12O7 • XHCl
Formula Weight
833.0
Purity
≥95%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/mlEthanol: 20 mg/ml
λmax
230, 299, 326 nm
SMILES
O=C(N[C@@H](CCCC)C(N[C@@H](CCCCN)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(NC1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)=O)=O)=O)C3=CC=CC=C3.Cl
InChi Code
InChI=1S/C41H60N12O7.ClH/c1-3-4-14-29(50-35(55)26-12-6-5-7-13-26)37(57)51-30(15-8-9-20-42)38(58)53-32(17-11-22-48-41(45)46)39(59)52-31(16-10-21-47-40(43)44)36(56)49-27-18-19-28-25(2)23-34(54)60-33(28)24-27;/h5-7,12-13,18-19,23-24,29-32H,3-4,8-11,14-17,20-22,42H2,1-2H3,(H,49,56)(H,50,55)(H,51,57)(H,52,59)(H,53,58)(H4,43,44,47)(H4,45,46,48);1H/t29-,30-,31-,32-;/m0./s1
InChi Key
DINNPVBPBDJKQJ-WYDLTDSDSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Bz-Nle-KRR-AMC is a fluorogenic peptide substrate for yellow fever virus (YFV) non-structural 3 (NS3) and dengue virus (DV) NS2B/3 serine proteases.1,2 Upon enzymatic cleavage by YFV NS3 or DV NS2B/3 serine proteases, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify YFV NS3 or DV NS2B/3 serine protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ulanday, G.E., Okamoto, K., and Morita, K. Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease. Trop. Med. Health 44:22, (2016).

    2. Löhr, K., Knox, J.E., Phong, W.Y., et al. Yellow fever virus NS3 protease: Peptide-inhibition studies. J. Gen. Virol. 88(Pt 8), 2223-2227 (2007).