A biotinylated peptide fragment of histone H3
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Histone H3K27Me2 (21-44)-GK-biotin (trifluoroacetate salt)

Item No. 27766

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
L-alanyl-L-threonyl-L-lysyl-L-alanyl-L-alanyl-L-arginyl-N6,N6-dimethyl-L-lysyl-L-seryl-L-alanyl-L-prolyl-L-alanyl-L-threonylglycylglycyl-L-valyl-L-lysyl-L-lysyl-L-prolyl-L-histidyl-L-arginyl-L-tyrosyl-L-arginyl-L-prolylglycylglycyl-L-lysyine-biotin, trifluoroacetate salt
Synonyms
  • ATKAAR-K(Me2)-SAPATGGVKKPHRYRPG-GK(Biotin)
  • Histone H3 (21-24) (Lys27me2)-GK(Biotin)
  • [Lys(Me2)27]-Histone H3 (21-44)-GK(Biotin) (trifluoroacetate salt)
Molecular Formula
C129H218N44O33S • XCF3COOH
Formula Weight
2945.5
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: 1 mg/ml
SMILES
O=C(O)C(F)(F)F.O=C(NCCCC[C@@H](C(O)=O)NC(CNC(CNC([C@@H]1CCCN1C([C@H](CCCNC(N)=N)NC([C@@H](NC([C@H](CCCNC(N)=N)NC([C@@H](NC([C@@H]2CCCN2C([C@H](CCCCN)NC([C@H](CCCCN)NC([C@H](C(C)C)NC(CNC(CNC([C@@]([C@@H](C)O)([H])NC([C@@H](NC([C@@H]3CCCN3C([C@@H](NC([C@H](CO)NC([C@H](CCCCN(C)C)NC([C@H](CCCNC(N)=N)NC([C@@H](NC([C@@H](NC([C@H](CCCCN)NC([C@@]([C@@H](C)O)([H])NC([C@@H](N[H])C)=O)=O)=O)C)=O)C)=O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)=O)=O)=O)=O)CC4=CN=CN4)=O)=O)CC5=CC=C(O)C=C5)=O)=O)=O)=O)=O)CCCC[C@@H]6SC[C@@](N7)([H])[C@]6([H])NC7=O
InChi Code
InChI=1S/C129H218N44O33S.C2HF3O2/c1-68(2)100(166-99(182)64-146-97(180)62-148-119(198)101(74(8)175)168-107(186)72(6)152-117(196)92-39-28-55-171(92)122(201)73(7)153-115(194)89(65-174)164-112(191)80(32-18-23-54-170(10)11)156-109(188)82(35-24-51-142-126(134)135)155-106(185)71(5)150-105(184)70(4)151-108(187)79(30-14-19-47-130)159-121(200)102(75(9)176)167-104(183)69(3)133)120(199)158-81(31-15-20-48-131)110(189)160-84(33-16-21-49-132)123(202)173-57-29-40-93(173)118(197)163-88(59-77-60-140-67-149-77)114(193)157-83(36-25-52-143-127(136)137)111(190)162-87(58-76-43-45-78(177)46-44-76)113(192)161-85(37-26-53-144-128(138)139)124(203)172-56-27-38-91(172)116(195)147-61-96(179)145-63-98(181)154-86(125(204)205)34-17-22-50-141-95(178)42-13-12-41-94-103-90(66-207-94)165-129(206)169-103;3-2(4,5)1(6)7/h43-46,60,67-75,79-94,100-103,174-177H,12-42,47-59,61-66,130-133H2,1-11H3,(H,140,149)(H,141,178)(H,145,179)(H,146,180)(H,147,195)(H,148,198)(H,150,184)(H,151,187)(H,152,196)(H,153,194)(H,154,181)(H,155,185)(H,156,188)(H,157,193)(H,158,199)(H,159,200)(H,160,189)(H,161,192)(H,162,190)(H,163,197)(H,164,191)(H,166,182)(H,167,183)(H,168,186)(H,204,205)(H4,134,135,142)(H4,136,137,143)(H4,138,139,144)(H2,165,169,206);(H,6,7)/t69-,70-,71-,72-,73-,74+,75+,79-,80-,81-,82-,83-,84-,85-,86-,87-,88-,89-,90-,91-,92-,93-,94-,100-,101-,102-,103-;/m0./s1
InChi Key
YZFIWSAVPWULPA-VUDJXMGVSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    250 µg

    $202

    Add
    Product Description

    Histone H3K27Me2 (21-44)-GK-biotin is a peptide fragment of histone H3 that corresponds to amino acid residues 22-45 of the human histone H3.1 and 3.2 sequences. It is dimethylated at lysine 27 and biotinylated via a C-terminal GK linker. Dimethylation of H3K27 is present on large chromatin domains across approximately 70% of total histone H3 and prevents firing of non-cell type-specific enhancers.1 H3K27Me2 accumulates in gene bodies of genes with low levels of expression in mouse embryonic stem cells. Histone H3K27Me2 (21-44)-GK-biotin has been used as a positive control in a high-throughput screen for inhibitors of the histone demethylase jumonji AT-rich interactive domain 1B (JARID1B).2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ferrari, K.J., Scelfo, A., Jammula, S., et al. Polycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity. Mol. Cell 53(1), 49-62 (2014).

    2. Sayegh, J., Cao, J., Zou, M.R., et al. Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen. The Journal of Biological Chemisty 288(13), 9408-9417 (2013).