A fluorescent probe for the detection of NO
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DAR-4M

Item No. 27897

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
9-[3-amino-2-carboxy-4-(methylamino)phenyl]-3,6-bis(dimethylamino)-xanthylium, inner salt
CAS Number
339527-79-6
Synonyms
  • DAF-4M
  • Diaminorhodamine-4M
Molecular Formula
C25H26N4O3
Formula Weight
430.5
Purity
≥95%
Emission
572 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble
SMILES
CNC1=C(N)C(C([O-])=O)=C(C(C(C=CC(N(C)C)=C2)=C2O3)=C4C3=C/C(C=C4)=[N+](C)/C)C=C1
InChi Code
InChI=1S/C25H26N4O3/c1-27-19-11-10-18(23(24(19)26)25(30)31)22-16-8-6-14(28(2)3)12-20(16)32-21-13-15(29(4)5)7-9-17(21)22/h6-13H,26H2,1-5H3,(H,30,31)
InChi Key
SEHXCXYNFKCWQY-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 mg

    $274

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    Product Description

    DAR-4M is a rhodamine-based photostable fluorescent probe for the detection of nitric oxide (NO).1 The fluorescence intensity of DAR-4M increases with increasing NO concentration, however, the fluorescent yield can be increased further in the presence of both NO and other reactive nitrogen species (RNS), but not in the presence of non-NO RNS alone.1,2 Upon oxidation, DAR-4M is converted to the triazole, DAR-4M T, which displays absorption/emission maxima of 554 and 572 nm, respectively.1,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kojima, H., Hirotani, M., Nakatsubo, N., et al. Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore. Anal. Chem. 73(9), 1967-1973 (2001).

    2. Lacza, Z., Horváth, E.M., Pankotai, E., et al. The novel red-fluorescent probe DAR-4M measures reactive nitrogen species rather than NO. J. Pharmacol. Toxicol. Methods 52(3), 335-340 (2005).

    3. Ostera, G., Tokumasu, F., Oliveira, F., et al. Plasmodium falciparum: Food vacuole localization of nitric oxide-derived species in intraerythrocytic stages of the malaria parasite. Exp. Parasitol. 120(1), 29-38 (2008).