A reagent used for tyramide signal amplification
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Biotinyl Tyramide

Item No. 27997

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(3aS,4S,6aR)-hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
CAS Number
41994-02-9
Molecular Formula
C18H25N3O3S
Formula Weight
363.5
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:5): 0.16 mg/ml
λmax
225, 280 nm
SMILES
OC1=CC=C(CCNC(CCCC[C@H]2[C@](NC(N3)=O)([H])[C@]3([H])CS2)=O)C=C1
InChi Code
InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
InChi Key
VZWXNOBHWODXCW-ZOBUZTSGSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $36

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    25 mg

    $81

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    50 mg

    $135

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    Product Description

    Biotinyl tyramide is a reagent that has been used for tyramide signal amplification (TSA) via catalyzed reporter deposition (CARD).1 In CARD, a reporter enzyme, such as horseradish peroxidase (HRP) conjugated to a secondary antibody, is bound to the target of interest and catalyzes the covalent deposition of biotinyl tyramide to the sample. The sample is then probed by a detector, such as streptavidin-HRP, allowing detection via chromogenic or fluorescent methods. Biotinyl tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bobrow, M.N., Litt, G.J., Shaughnessy, K.J., et al. The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats. J. Immunol. Methods 150(1-2), 145-149 (1992).

    2. Kadkol, S.S., Gage, W.R., and Pasternack, G.R. In situ hybridization-Theory and practice. Mol. Diagn. 4(3), 169-183 (1999).