A fluorogenic substrate for β-galactosidase
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Resorufin β-D-Galactopyranoside

Item No. 28015

Technical Information
Formal Name
7-(β-D-galactopyranosyloxy)-3H-phenoxazin-3-one
CAS Number
95079-19-9
Molecular Formula
C18H17NO8
Formula Weight
Purity
≥98%
Emission
580 nm
Excitation
570 nm
A solid
DMF: 2 mg/mlDMSO: 25 mg/mlEthanol: 1 mg/mlPBS (pH 7.2): 0.3 mg/ml
λmax
245, 458 nm
SMILES
O=C1C=CC2=NC(C=CC(O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)=C4)=C4OC2=C1
InChi Code
InChI=1S/C18H17NO8/c20-7-14-15(22)16(23)17(24)18(27-14)25-9-2-4-11-13(6-9)26-12-5-8(21)1-3-10(12)19-11/h1-6,14-18,20,22-24H,7H2/t14-,15+,16+,17-,18-/m1/s1
InChi Key
QULZFZMEBOATFS-DISONHOPSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Resorufin β-D-galactopyranoside is a fluorogenic substrate for β-galactosidase.1 Upon enzymatic cleavage by β-galactosidase, the fluorescent moiety resorufin is released and its fluorescence can be used to quantify β-galactosidase activity. Resorufin displays excitation/emission maxima of 570/580 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hofmann, J., and Sernetz, M. Immobilized enzyme kinetics analyzed by flow-through microfluorimetry: Resorufin-β-D-galactopyranoside as a new fluorogenic substrate for β-galactosidase. Anal. Chim. Acta 163, 67-72 (1984).