A biotinylated peptide fragment of histone H4
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Histone H4R3Me2s (1-21)-GGK-biotin (trifluoroacetate salt)

Item No. 28192

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N2-(N2-acetyl-L-serylglycyl-Nω,Nω'-dimethyl-L-arginylglycyl-L-lysylglycylglycyl-L-lysylglycyl-L-leucylglycyl-L-lysylglycylglycyl-L-alanyl-L-lysyl-L-arginyl-L-histidyl-L-arginyl-L-lysyl-L-valylglycylglycyl)-N6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl)-L-lysine, trifluoroacetate salt
Synonyms
  • Ac-SG-R(me2s)-GKGGKGLGKGGAKRHRKV-GGK(Biotin)
  • [Arg(Me2s)3]-Histone H4 (1-21)-GGK(Biotin)
  • Histone H4 (1-21) (Arg3me2s)
Molecular Formula
C111H197N43O29S • XCF3COOH
Formula Weight
2630.1
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: 1 mg/ml
SMILES
[H]N(C(C)=O)[C@@H](CO)C(NCC(N[C@@H](CCCNC(N(C)C)=N)C(NCC(N[C@@H](CCCCN)C(NCC(NCC(N[C@@H](CCCCN)C(NCC(N[C@@H](CC(C)C)C(NCC(N[C@@H](CCCCN)C(NCC(NCC(N[C@H](C(N[C@@H](CCCCN)C(N[C@@H](CCCNC(N)=N)C(N[C@H](C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCCN)C(N[C@@H](C(C)C)C(NCC(NCC(N[C@@H](CCCCNC(CCCC[C@@H]1SC[C@@](N2)([H])[C@]1([H])NC2=O)=O)C(O)=O)=O)=O)=O)=O)=O)=O)CC3=CN=CN3)=O)=O)=O)C)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.O=C(O)C(F)(F)F
InChi Code
InChI=1S/C111H197N43O29S.C2HF3O2/c1-62(2)46-76(145-91(167)58-133-97(171)69(28-13-19-39-114)140-86(162)53-128-83(159)50-131-95(169)67(26-11-17-37-112)141-88(164)55-132-98(172)70(32-25-45-126-110(121)154(7)8)143-90(166)57-135-100(174)78(59-155)139-65(6)156)99(173)134-56-89(165)142-68(27-12-18-38-113)96(170)130-49-82(158)127-52-85(161)138-64(5)94(168)146-71(29-14-20-40-115)101(175)148-74(34-24-44-125-109(119)120)103(177)150-77(47-66-48-122-61-137-66)105(179)149-73(33-23-43-124-108(117)118)102(176)147-72(30-15-21-41-116)104(178)152-92(63(3)4)106(180)136-51-84(160)129-54-87(163)144-75(107(181)182)31-16-22-42-123-81(157)36-10-9-35-80-93-79(60-184-80)151-111(183)153-93;3-2(4,5)1(6)7/h48,61-64,67-80,92-93,155H,9-47,49-60,112-116H2,1-8H3,(H2,121,126)(H,122,137)(H,123,157)(H,127,158)(H,128,159)(H,129,160)(H,130,170)(H,131,169)(H,132,172)(H,133,171)(H,134,173)(H,135,174)(H,136,180)(H,138,161)(H,139,156)(H,140,162)(H,141,164)(H,142,165)(H,143,166)(H,144,163)(H,145,167)(H,146,168)(H,147,176)(H,148,175)(H,149,179)(H,150,177)(H,152,178)(H,181,182)(H4,117,118,124)(H4,119,120,125)(H2,151,153,183);(H,6,7)/t64-,67-,68-,69-,70-,71-,72-,73-,74-,75-,76-,77-,78-,79-,80-,92-,93-;/m0./s1
InChi Key
GWMACEGDYIRHQH-GCCXERLZSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    250 µg

    $303

    Add
    Product Description

    Histone H4R3Me2s (1-21)-GGK-biotin is a peptide fragment of histone H4 that corresponds to amino acid residues 2-22 of the human H4 sequence. It is symmetrically dimethylated at arginine 3 and biotinylated via a C-terminal GGK linker. Symmetric dimethylation of histone 4 at arginine 3 is associated with transcriptional repression.1 It can recruit the DNA methyltransferase DNMT3a, leading to DNA methylation and further transcriptional repression. Histone H4R3Me2s (1-21)-GGK-biotin has been used as a substrate for the qualitative measurement of jumonji domain containing 1B (JMJD1B) arginine demethylase activity and in dot blot assays to verify antibody specificity.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Blanc, R.S., and Richard, S. Arginine methylation: The coming of age. Mol. Cell 65(1), 8-24 (2017).

    2. Li, S., Ali, S., Duan, X., et al. JMJD1B demethylates H4R3me2s and H3K9me2 to facilitate gene expression for development of hematopoietic stem and progenitor cells. Cell Rep. 23(2), 389-403 (2018).