YTHDC2 Polyclonal Antibody

YTH domain containing 2 (YTHDC2) is an ATP-dependent 3’-5’ RNA helicase and a member of the DExD/H-box family of helicases.¹ YTHDC2 contains ATP binding, ATP hydrolysis, and RNA binding motifs.¹ The aYTH domain binds to N⁶-methyladenosine-modified germline messenger RNA to regulate meiotic gene expression in mammalian germ cells, mediating the mitotic-to-meiotic transition in mouse germ cells.^2,3 Ythdc2^-/- male and female mice show disrupted meiotic prophase progression.³ Male Ythdc2^-/- mice have degraded germ cells and lack mature spermatozoa, while female Ythdc2^-/- mice lack developing ovarian follicles and have few germ cells. YTHDC2 also facilitates hepatitis C virus (HCV) genome replication by binding to the HCV replication cofactor cyclophilin B and the HCV RNA polymerase non-structural protein 5B (NS5B).⁴ YTHDC2 downregulation inhibits proliferation in Huh7 hepatocellular carcinoma cells and reduces metastasis a Y2KD-116 mouse xenograft model using HCT116 cells in which YTHDC2 is downregulated.^4,5 The expression of YTHDC2 in isolated human tumor tissue is positively correlated with tumor stage and lymph node metastasis.⁵ Cayman’s YTHDC2 Polyclonal Antibody can be used for immunofluorescence, immunohistochemistry, and Western blot applications. The antibody recognizes YTHDC2 at 160 kDa from human samples.

WARNING This product is not for human or veterinary use.

1. Tanabe, A., Konno, J., Tanikawa, K., et al. Transcriptional machinery of TNF-α-inducible YTH domain containing 2 (YTHDC2) gene. Gene 535(1), 24-32 (2014).

2. Wojtas, M.N., Pandey, R.R., Mendel, M., et al. Regulation of m6A transcripts by the 3'→5' RNA helicase YTHDC2 is essential for a successful meiotic program in the mammalian germline. Mol. Cell 68(2), 374-387 (2017).

3. Bailey, A.S., Batista, P.J., Gold, R.S., et al. The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. Elife pii:e26116, (2017).

4. Morohashi, K., Sahara, H., Watashi, K., et al. Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B. PLoS One 6(4):e18285, (2011).

5. Tanabe, A., Tanikawa, K., Tsunetomi, M., et al. RNA helicase YTHDC2 promotes cancer metastasis via the enhancement of the efficiency by which HIF-1α mRNA is translated. Cancer Lett. 376(1), 34-42 (2016).