A hedgehog pathway inhibitor
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HhAntag

Item No. 28296

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[4-chloro-3-[6-(dimethylamino)-1H-benzimidazol-2-yl]phenyl]-3,5-dimethoxy-benzamide
CAS Number
496794-70-8
Molecular Formula
C24H23ClN4O3
Formula Weight
450.9
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mLDMSO: 2 mg/mLDMSO:PBS (pH 7.2) (1:2): 0.3mg/mLEthanol: 1 mg/mL
λmax
213 nm
SMILES
CN(C)C1=CC=C2C(NC(C3=CC(NC(C4=CC(OC)=CC(OC)=C4)=O)=CC=C3Cl)=N2)=C1
InChi Code
InChI=1S/C24H23ClN4O3/c1-29(2)16-6-8-21-22(12-16)28-23(27-21)19-11-15(5-7-20(19)25)26-24(30)14-9-17(31-3)13-18(10-14)32-4/h5-13H,1-4H3,(H,26,30)(H,27,28)
InChi Key
UBHJFPVTEATUFS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    5 mg

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    10 mg

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    25 mg

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    Cayman Chemical
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    Product Description

    HhAntag is an inhibitor of the Sonic Hedgehog (Shh) pathway (EC50 = 0.4 μM for Shh signaling in a reporter assay).1 It inhibits proliferation of GNP cells when used at concentrations ranging from 0.1 nM to 25 μM. In vivo, HhAntag (20 or 100 mg/kg) suppresses Gli1 mRNA levels, a target of Shh signaling, and increases tumor cell death in a mouse model of Ptc1+/-p53-/- medulloblastoma. HhAntag also blocks basal and bone morphogenic protein 2-induced chondrogenesis in micromass cultures of mouse limb mesenchymal cells.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Romer, J.T., Kimura, H., Magdaleno, S., et al. Suppression of the Shh pathway using a small molecule inhibitor eliminates medulloblastoma in Ptc1+/-p53-/- mice. Cancer Cell 6(3), 229-240 (2004).

    2. Mundy, C., Bello, A., Sgariglia, F., et al. HhAntag, a hedgehog signaling antagonist, suppresses chondrogenesis and modulates canonical and non-canonical BMP signaling. J. Cell Physiol. 231(5), 1033-1044 (2016).