Globotetraosylceramide Polyclonal Antibody

Globotetraosylceramides (Item No. 24881) are bioactive neutral glycosphingolipids. They are the major glycolipids in human erythrocytes.¹ They act as receptors for the Shiga toxins Stx1, Stx2, and Stx2e, the cytotoxic protein pierisin-1, and parvovirus B19.^2,3,4 Globotetraosylceramides increase the expression of proteins responsible for enamel deposition, including ameloblastin, amelogenin, and enamelin, in dental epithelial cells and activate the ERK and p38 MAPK signaling pathways.⁵ Levels of globotetraosylceramides are elevated in fibroblasts from patients with salt and pepper syndrome, a neurocutaneous condition characterized by intellectual disability and hyper- and hypo-pigmented skin.⁶ Globotetraosylceramide Polyclonal Antibody can be used for ELISA and TLC immunoblotting. [Matreya, LLC. Catalog No. 1960]

WARNING This product is not for human or veterinary use.

1. Yamakawa, T., Yokoyama, S., and Kiso, N. Structure of main globoside of human erythrocytes. J. Biochem. 52(3), 228-229 (1962).

2. Müthing, J., Scheweppe, C.H., Karch, H., et al. Shiga toxins, glycosphingolipid diversity, and endothelial cell injury. Thromb. Haemost. 101(2), 252-264 (2009).

3. Matsushima-Hibiya, Y., Watanabe, M., Hidari, J.I.-P.J., et al. Identification of glycosphingolipid receptors for pierisin-1, a guanine-specific ADP-ribosylating toxin from the cabbage butterfly. The Journal of Biological Chemisty 278(11), 9972-9978 (2003).

4. Nasir, W., Nilsson, J., Ologsson, S., et al. Parvovirus B19 VLP recognizes globoside in supported lipid bilayers. Virology 456-457, 364-369 (2014).

5. Nakamura, T., Chiba, Y., Naruse, M., et al. Globoside accelerates the differentiation of dental epithelial cells into ameloblasts. Int. J. Oral Sci. 8(4), 205-212 (2016).

6. Boccuto, L., Aoki, K., Flanagan-Steet, H., et al. A mutation in a ganglioside biosynthetic enzyme, ST3GAL5, results in salt & pepper syndrome, a neurocutaneous disorder with altered glycolipid and glycoprotein glycosylation. Hum. Mol. Genet. 23(2), 418-433 (2014).