A lipophilic, amine-reactive fluorescent probe
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BODIPY 503/512

Item No. 28800

Technical Information
Formal Name
(T-4)-[5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-propanoato(2-)-κN1]difluoro-borate(1-), monohydrogen
CAS Number
165599-63-3
Synonyms
  • BDP FL
  • 3-BODIPY-Propanoic Acid
Molecular Formula
C14H15BF2N2O2
Formula Weight
Purity
≥95%
Emission
512 nm
Excitation
503 nm
A solid
Methanol: soluble
SMILES
OC(CCC1=CC=C(C=C(C(C)=C2)[N+]3=C2C)[N]1[B-]3([F])[F])=O
InChi Code
InChI=1S/C14H15BF2N2O2/c1-9-7-10(2)18-13(9)8-12-4-3-11(5-6-14(20)21)19(12)15(18,16)17/h3-4,7-8H,5-6H2,1-2H3,(H,20,21)
InChi Key
BJDJEJIINKBPHY-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    BODIPY 503/512 is a lipophilic, amine-reactive fluorescent probe.1 It has been used to label oligonucleotide probes and primers for the quantitation of DNA and RNA by PCR and to monitor the uptake and trafficking of BODIPY-labeled proteins and other compounds within cells by fluorescence microscopy.2,3,4,1 BODIPY 503/512 has also been used to identify and sort adipocytes from mouse white and brown adipose tissue by flow cytometry.5 It displays excitation/emission maxima of 503/512 nm, respectively.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Murakami, M., Cabral, H., Matsumoto, Y., et alImproving drug potency and efficacy by nanocarrier-mediated subcellular targeting. Sci. Transl. Med. 3(64), 64ra62 (2011).

    2. Kurata, S., Kanagawa, T., Yamada, K., et alFluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY® FL-labeled probe or primer. Nucleic Acids Res. 29(6), E34 (2001).

    3. Bittel, A.M., Saldivar, I.S., Dolman, N.J., et alSuperresolution microscopy with novel BODIPY-based fluorophores. PLoS One 13(10), e0206104 (2018).

    4. Surewaard, B.G.J., Deniset, J.F., Zemp, F.J., et alIdentification and treatment of the Staphylococcus aureus reservoir in vivo. J. Exp. Med. 213(7), 1141-1151 (2016).

    5. Hagberg, C.E., Li, Q., Kutschke, M., et alFlow cytometry of mouse and human adipocytes for the analysis of browning and cellular heterogeneity. Cell Rep. 24(10), 2746-2756 (2018).