A fluorogenic substrate for caspase-4
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Ac-LEVD-AFC

Item No. 28809

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-leucyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine
CAS Number
1092077-23-0
Synonyms
  • Ac-Leu-Glu-Val-Asp-AFC
  • Ac-Leu-Glu-Val-Asp-7-amino-4-trifluormethylcoumarin
Molecular Formula
C32H40F3N5O11
Formula Weight
727.7
Purity
≥95%
Emission
505 nm
Excitation
400 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 10 mg/ml
λmax
234, 340 nm
SMILES
CC(N[C@@H](CC(C)C)C(N[C@@H](CCC(O)=O)C(N[C@@H](C(C)C)C(N[C@@H](CC(O)=O)C(NC1=CC=C(C(C(F)(F)F)=CC(O2)=O)C2=C1)=O)=O)=O)=O)=O
InChi Code
InChI=1S/C32H40F3N5O11/c1-14(2)10-21(36-16(5)41)30(49)38-20(8-9-24(42)43)28(47)40-27(15(3)4)31(50)39-22(13-25(44)45)29(48)37-17-6-7-18-19(32(33,34)35)12-26(46)51-23(18)11-17/h6-7,11-12,14-15,20-22,27H,8-10,13H2,1-5H3,(H,36,41)(H,37,48)(H,38,49)(H,39,50)(H,40,47)(H,42,43)(H,44,45)/t20-,21-,22-,27-/m0/s1
InChi Key
FETZKXHXYOHAAN-LFYAFONDSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    Product Description

    Ac-LEVD-AFC is a fluorogenic substrate for caspase-4.1,2 Upon enzymatic cleavage by caspase-4, 7-amino-4-trifluoromethylcoumarin (AFC) is released and its fluorescence can be used to quantify caspase-4 activity. AFC displays excitation/emission maxima of 400/505 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Koehn, S., Trueck, M., Poerhlmann, T.G., et al. Assessment of caspase-4 released free AFC by RP-HPLC and fluorescence detection. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 874(1-2), 111-114 (2008).

    2. Pallett, M.A., Crepin, V.F., Serafini, N., et al. Bacterial virulence factor inhibits caspase-4/11 activation in intestinal epithelial cells. Mucosal Immunol. 10(3), 602-612 (2017).