smgGDS-607 Monoclonal Antibody (Clone 4D6)

SmgGDS is a chaperone protein and guanine nucleotide exchange factor encoded by RAP1GDS1 that regulates the prenylation and trafficking of small GTPases and has a role in oncogenesis.^1,2,3 Alternative splicing of the RAP1GDS1 pre-mRNA produces a full-length isoform, smgGDS-607, and a short isoform, smgGDS-558.¹ SmgGDS-607 is composed of 607 residues and contains an N-terminal nuclear export sequence and multiple armadillo repeats, which are essential for binding to small GTPases that contain a C-terminal polybasic region.^1,2,4 It is expressed in a variety of tissues and cancer cell lines.² SmgGDS-607 binds to newly synthesized, preprenylated small GTPases and traffics them to prenyltransferases (PTases), which catalyze their prenylation, a process that is critical for the membrane localization and subsequent activation of small GTPases.^2,3 An elevated ratio of smgGDS-607 to smgGDS-558 has been observed in cancer cell lines and is associated with reduced survival in patients with breast cancer.² Cayman's smgGDS-607 Monoclonal Antibody can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes a unique region of smgGDS-607 that is not present in smgGDS-558, specifically detecting smgGDS-607 at ~60 kDa from human and African green monkey samples.

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1. Gonyo, P., Bergom, C., Brandt, A.C., et al. SmgGDS is a transient nucleolar protein that protects cells from nucleolar stress and promotes the cell cycle by regulating DREAM complex gene expression. Oncogene 36(50), 6873-6883 (2017).

2. Brandt, A.C., McNally, L.R., Lorimer, E.L., et al. Splice switching an oncogenic ratio of SmgGDS isoforms as a strategy to diminish malignancy. Proc. Natl. Acad. Sci. USA 117(7), 3627-3636 (2020).

3. Schuld, N.J., Vervacke, J.S., Lorimer, E.L., et al. The chaperone protein SmgGDS interacts with small GTPases entering the prenylation pathway by recognizing the last amino acid in the CAAX motif. The Journal of Biological Chemisty 289(10), 6862-6876 (2014).

4. Berg, T.J., Gastonguay, A.J., Lorimer, E.L., et al. Splice variants of SmgGDS control small GTPase prenylation and membrane localization. The Journal of Biological Chemisty 285(46), 35255-35266 (2010).