An amine reactive reagent
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Biotin-PEG4-NHS ester

Item No. 29114

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-17-oxo-4,7,10,13-tetraoxa-16-azaheneicosanoic acid, 2,5-dioxo-1-pyrrolidinyl ester
CAS Number
459426-22-3
Synonyms
  • NHS-PEO4-Biotin
  • NHS-PEG4-Biotin
Molecular Formula
C25H40N4O10S
Formula Weight
588.7
Purity
≥90%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 30 mg/mlPBS (pH 7.2): 5 mg/ml
SMILES
O=C1N[C@]2([H])[C@H](CCCCC(NCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O)=O)SC[C@]2([H])N1
InChi Code
InChI=1S/C25H40N4O10S/c30-20(4-2-1-3-19-24-18(17-40-19)27-25(34)28-24)26-8-10-36-12-14-38-16-15-37-13-11-35-9-7-23(33)39-29-21(31)5-6-22(29)32/h18-19,24H,1-17H2,(H,26,30)(H2,27,28,34)/t18-,19-,24-/m0/s1
InChi Key
DTLVBHCSSNJCMJ-JXQFQVJHSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    25 mg

    $72

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    50 mg

    $134

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    100 mg

    $252

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    Product Description

    Biotin-PEG4-NHS ester is an amine reactive reagent that contains biotin linked via a four-unit PEG spacer to N-hydroxysuccinimide (NHS), which facilitates the coupling of biotin to primary amines.1 Biotin-PEG4-NHS ester has been used in the biotinylation of proteins and antibodies.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Smith, G.P. Kinetics of amine modification of proteins. Bioconjug. Chem. 17(2), 501-506 (2006).

    2. Evison, B.J., Palmer, J.T., Lambert, G., et al. A small molecule inhibitor of PCSK9 that antagonizes LDL receptor binding via interaction with a cryptic PCSK9 binding groove. Bioorg. Med. Chem. 28(6), 115344 (2020).

    3. Rajendran, M., Sun, W., Comella, P., et al. An immuno-assay to quantify inflyenza virus hemagglutinin with correctly folded stalk domains in vaccine preparations. PLoS ONE 13(4), e0194830 (2018).