For immunochemical detection of METTL16
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Measurement and Detection Tool(s)
26343METTL16 (human, recombinant)
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METTL16 Monoclonal Antibody (Clone 4C9)

Item No. 29207

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • N6-Adenosine-methyltransferase METTL16
  • EC 2.1.1.346
  • EC 2.1.1.62
  • Methyltransferase-like Protein 16
  • Methyltransferase 10 Domain-containing Protein
  • Putative Methyltransferase METT10D
  • U6 snRNA Methyltransferase METT10D
  • U6 Small Nuclear RNA (adenine-(43)-N(6))-Methyltransferase
Immunogen
Full-length recombinant human METTL16
Clone Designation
4C9
Formulation (Request formulation change)
100 µg of protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA, IF, and WB
Species Reactivity
(+) Human; other species not tested
UniProt Accession №
Q86W50
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    100 µg

    $347

    Add
    Cayman Chemical
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    Product Description

    Methyltransferase-like protein 16 (METTL16) is an m6A RNA methyltransferase encoded by the METTL16 gene in humans and localized to the cell nucleus.1,2 It is composed of a methyltransferase domain that associates with RNA and two vertebrate conserved regions (VCRs) on the C-terminal end that promote splicing.3 METTL16 acts as an epigenetic writer by methylating the pre-mRNA of the S-adenosylmethionine (SAM) synthetase MAT2A in the presence of SAM. It also methylates the adenine at position 43 of spliceosome component U6 snRNA and interacts with cancer-associated MALAT1 long non-coding RNA (lncRNA).4,1 Knockout of Mettl16 in mouse embryos leads to a reduction in Mat2a mRNA expression, transcriptome dysregulation, and lethality at approximately the implantation stage.5 The expression of METTL16 is increased in isolated human colon, but not rectal, adenocarcinoma tumor tissue, and decreased METTL16 expression in rectal adenocarcinoma tumor tissue is associated with lower overall survival.6 Cayman’s METTL16 Monoclonal Antibody (Clone 4C9) can be used for ELISA, Immunofluorescence, and Western blot applications. The antibody recognizes METTL16 at 64 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ruszkowska, A., Ruszkowski, A., Dauter, Z., et al. Structural insights into the RNA methyltransferase domain of METTL16. Sci. Rep. 8(1), 5311 (2018).

    2. Brown, J.A., Kinzig, C.G., DeGregorio, S.J., et al. Methyltransferase-like protein 16 binds the 3'-terminal triple helix of MALAT1 long noncoding RNA. Proc. Natl. Acad. Sci. USA 113(49), 14013-14018 (2016).

    3. Pendleton, K.E., Chen, B., Liu, K., et al. The U6 snRNA m6A methyltransferase METTL16 regulates SAM synthetase intron retention. Cell 169(5), 824-835 (2017).

    4. Warda, A.S., Kretschmer, J., Hackert, P., et al. Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs. EMBO Rep. 18(11), 2004-2014 (2017).

    5. Mendel, M., Chen, K.M., Homolka, D., et al. Methylation of structured RNA by the m6A writer METTL16 is essential for mouse embryonic development. Mol. Cell. 71(5), 986-1000 (2018).

    6. Liu, X., Liu, L., Dong, Z., et al. Expression patterns and prognostic value of m6A-related genes in colorectal cancer. Am. J. Transl. Res. 11(7), 3972-3991 (2019).