A chemical activator of DNA and RNA synthesis
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Product Type

Biochemicals
Lab Reagents

Research Area

Cell Biology

Saccharin 1-methylimidazole

Item No. 29242

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
benzo[d]isothiazol-3(2H)-one 1,1-dioxide, 1-methyl-1H-imidazole
CAS Number
482333-74-4
Molecular Formula
C7H5NO3S • C4H6N2
Formula Weight
265.3
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 5mg/mLDMSO: 5mg/mLEthanol: 10mg/mLPBS (pH 7.2): 10mg/mL
SMILES
O=S1(C2=CC=CC=C2C(N1)=O)=O.CN3C=CN=C3
InChi Code
InChI=1S/C7H5NO3S.C4H6N2/c9-7-5-3-1-2-4-6(5)12(10,11)8-7;1-6-3-2-5-4-6/h1-4H,(H,8,9);2-4H,1H3
InChi Key
DMSNKRPEUQVMNF-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $63

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    10 mg

    $110

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    25 mg

    $242

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    50 mg

    $423

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    Product Description

    Saccharin 1-methylimidazole is a chemical activator that has been commonly used in solid-phase synthesis of DNA and RNA oligonucleotides.1 It catalyzes the phosphoramidite coupling reaction of the 5' hydroxy of immobilized nucleosides and the amine group of nucleoside phosphoramidite. Saccharin 1-methylimidazole has been used to synthesize single-stranded DNA (ssDNA) oligonucleotides that are resistant to nuclease digestion.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Russell, M.A., Laws, A.P., Atherton, J.H., et al. The mechanism of the phosphoramidite synthesis of polynucleotides. Org. Biomol. Chem. 6(18), 3270-3275 (2008).

    2. Tatro, E.T., Gianneschi, N., and Rush, A.M. Methods for arranging and packing nucleic acids for unusual resistance to nucleases and targeted delivery for gene therapy. (2015).