For immunochemical detection of α-synuclein (phospho-Ser129)
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α-Synuclein (Phospho-Ser129) Polyclonal Antibody

Item No. 29252

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SNCA
  • α-Syn
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding phospho-Ser129 of rat α-synuclein
MW
~15 kDa
Formulation (Request formulation change)
100 µl of Affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg BSA per ml, and 50% glycerol
Host
Rabbit
Applications
IHC and WB
Species Reactivity
(+) Human(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $510

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    Product Description

    α-Synuclein is a 140-amino acid intracellular protein encoded by the SNCA gene in humans.1 It is primarily localized to the synaptic terminal of neurons in both the central and peripheral nervous systems where it has a role in regulation of synaptic vesicle docking and neurotransmitter release.2 α-Synuclein is an intrinsically disordered, multi-domain protein.1,3 It exists as monomer in the cytoplasm, however, mutations, posttranslational modifications, and changes in cellular environmental conditions can induce oligomerization and aggregate formation that lead to various pathologies, including Parkinson's disease, Lewy body disease, multiple system atrophy (MSA), and amyotrophic lateral sclerosis (ALS). Phosphorylation of α-synuclein at serine 129 (Ser129) is increased in response to mitochondrial stress and is reduced by lysosome inhibition in SH-SY5Y cells.4 It enhances α-synuclein-induced neurotoxicity in Drosophila and rat models of Parkinson's disease.5,4 Levels of phosphorylated Ser129 (phospho-Ser129) are increased in Lewy bodies in postmortem brains from patients with Parkinson's disease and Lewy body disease compared with control individuals.5,6,4 Cayman's α-Synuclein (Phospho-Ser129) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes α-synuclein (phospho-Ser129) at approximately 15 kDa from human, mouse, and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Villar-Piqué, A., da Fonseca, T.L., and Outeiro, T.F. Structure, function and toxicity of alpha-synuclein: The Bermuda triangle in synucleinopathies. J. Neurochem. 139(Suppl 1), 240-255 (2016).

    2. Rocha, E.M., De Miranda, B., and Sanders, L.H. Alpha-synuclein: Pathology, mitochondrial dysfunction and neuroinflammation in Parkinson’s disease. Neurobiol. Dis. 109(Pt B), 249-257 (2017).

    3. Bendor, J.T., Logan, T.P., and Edwards, R.H. The function of α-synuclein. Neuron 79(6), 1044-1066 (2013).

    4. Arawaka, S., Sato, H., Sasaki, A., et al. Mechanisms underlying extensive Ser129-phosphorylation in α-synuclein aggregates. Acta Neuropathol. Commun. 5(1), 48 (2017).

    5. Sato, H., Kato, T., and Arawaka, S. The role of Ser129 phosphorylation of α-synuclein in neurodegeneration of Parkinson’s disease: A review of in vivo models. Rev. Neurosci. 24(2), 115-123 (2013).

    6. Anderson, J.P., Walker, D.E., Goldstein, J.M., et al. Phosphorylation of Ser-129 is the dominant pathological modification of α-synuclein in familial and sporadic Lewy body disease. The Journal of Biological Chemisty 281(40), 29739-29752 (2006).