For immunochemical detection of CaMKIIα (phospho-Thr286) and CaMKIIβ (phospho-Thr286)
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CaMKII (Phospho-Thr286) Polyclonal Antibody

Item No. 29253

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Calcium/Calmodulin-dependent Protein Kinase II
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding phospho-Thr286 of rat CaM Kinase II
MW
~50 and 60 kDa for CaMKIIα phospho-Thr286 and CaMKIIβ phospho-Thr286, respectively
Formulation (Request formulation change)
100 µl of Affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
IHC, WB
Species Reactivity
(+) Human(+) Mouse(+) Rabbit(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $516

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    Product Description

    Calcium/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine protein kinase and member of the calcium/calmodulin-activated protein kinase family.1,2,3 There are four isoforms of CaMKII, α, β, γ, and δ, encoded by CaMK2A, CaMK2B, CaMK2G, and CaMK2D in humans.1 CaMKIIα and CaMKIIβ are predominantly expressed in neuronal tissue, while CaMKIIγ and CaMKIIδ are primarily found in cardiac tissue, however, the various isoforms of CaMKII can be found in nearly all cell types. Upon calcium binding, CaMKII undergoes a conformational switch that displaces the autoinhibitory domain inducing autophosphorylation of threonine 286 (Thr286) and autonomous activation of the kinase.4 Activation of CaMKII in dendritic spines in response to increased calcium concentration facilitates the induction of long term potentiation (LTP) and memory formation in rodents.5 Phospho-site mutant Camk2aT286A knock-in mice exhibit impaired LTP, as well as learning and memory deficits.5 Cayman’s CaMKII (Phospho-Thr286) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes CaMKIIα phospho-Thr286 and CaMKIIβ phospho-Thr286 at approximately 50 and 60 kDa, respectively, from human, mouse, rabbit, and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Beckendorf, J., van den Hoogenhof, M.M.G., and Backs, J. Physiological and unappreciated roles of CaMKII in the heart. Basic Res. Cardiol. 113(4):29, (2018).

    2. Hughes, K., Edin, S., Antonsson, Å., et al. Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IκB kinase. The Journal of Biological Chemisty 276(38), 36008-36013 (2001).

    3. Barria, A., Muller, D., Derkach, V., et al. Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation. Science 276(7), 2042-2045 (1997).

    4. Means, A.R. Regulatory cascades involving calmodulin-dependent protein kinases. Mol. Endocrinol. 14(1), 4-13 (2000).

    5. Chang, J.-Y., Parra-Bueno, P., Laviv, T., et al. CaMKII autophosphorylation is necessary for optimal integration of Ca2+ signals during LTP induction, but not maintenance. Neuron 94(4):800-808.e4, (2017).