For immunochemical detection of choline acetyltransferase
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Choline Acetyltransferase Polyclonal Antibody

Item No. 29255

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ChAT
Immunogen
Recombinant human choline acetyltransferase
MW
~70 kDa
Formulation (Request formulation change)
100 µl affinity-purified polyclonal antibody
Storage Buffer
PBS with 5 mg/ml BSA and 0.2% sodium azide
Host
Goat
Applications
IHC and WB
Species Reactivity
(+) Human(+) Avian(+) Chicken(+) Guinea Pig(+) Mouse(+) Non-human Primate(+) Rat
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $464

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    Product Description

    Choline acetyltransferase (ChAT) is an acyltransferase that catalyzes the synthesis of acetylcholine from acetyl coenzyme A (acetyl-CoA; Item No. 16160) and choline.1 It is composed of a catalytic domain flanked on both sides by a substrate binding domain.1,2 ChAT is synthesized in the soma of cholinergic neurons in the central and peripheral nervous system and is transported to nerve terminals where it exists in a soluble form or on synaptic vesicles in a membrane-bound form.1 It is used as a marker of cholinergic neurons.3 Genome-wide deletion of Chat in mice is perinatal lethal.4 Intracerebroventricular transplantation of human HB1.F3 neural stem cells overexpressing CHAT reverses learning and memory deficits in a mouse model of Alzheimer's disease induced by kainic acid (Item No. 78050).5 ChAT activity is reduced in a number of neurodegenerative disorders, including Alzheimer's disease, amyotrophic lateral sclerosis (ALS), and Huntington's disease.1 SNPs in CHAT are associated with Alzheimer's disease in humans.6 Cayman's Choline Acetyltransferase Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes ChAT at approximately 70 kDa from human, avian, chicken, guinea pig, mouse, non-human primate, and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Oda, Y. Choline acetyltransferase: The structure, distribution and pathologic changes in the central nervous system. Pathol. Int. 49(11), 921-937 (1999).

    2. Kim, A.-R., Rylett, R.J., and Shilton, B.H. Substrate binding and catalytic mechanism of human choline acetyltransferase. Biochem. 45(49), 14621-14631 (2006).

    3. Wu, D., and Hersh, L.B. Choline acetyltransferase: Celebrating its fiftieth year. J. Neurochem. 62(5), 1653-1663 (1994).

    4. Brandon, E.P., Lin, W., D'Amour, K.A., et al. Aberrant patterning of neuromuscular synapses in choline actyltransferase-deficient mice. J. Neurosci. 23(2), 539-549 (2003).

    5. Park, D., Joo, S.S., Kim, T.K., et al. Human neural stem cells overexpressing choline acetyltransferase restore cognitive function of kainic acid-induced learning and memory deficit animals. Cell Transplant. 21(1), 365-371 (2012).

    6. Yuan, H., Xia, Q., Ling, K.H.J., et al. Association of choline acetyltransferase gene polymorphisms (SNPs rs868750g/A, rs1880676G/A, rs2177369G/A and rs3810950G/A) with Alzheimer’s disease risk: A meta-analysis. PLoS Biol. 11(7), e0159022 (2016).