COBRA1 Monoclonal Antibody

Cofactor of BRCA1 (COBRA1), also known as negative elongation factor B (NELFB), is a 580-amino acid nuclear protein encoded by NELFB in humans.^1,2 It induces chromosome decondensation by targeting lac operator-containing chromosome regions and is a component of NELF, a four-subunit complex that suppresses RNA polymerase II elongation in vitro. COBRA1 protein levels are enriched in mammary gland luminal epithelium, and it has a role in modulating estrogen-dependent and -independent expression of oncogenes in various breast cancer cell lines.² Ectopic expression or knockdown of NELFB suppresses or accelerates breast cancer cell growth, respectively, as well as enhances androgen-mediated transcription.^2,3 COBRA1 also interacts with the activated protein-1 (AP-1) family members c-Jun and c-Fos, and inhibits AP-1-mediated transcription in vitro.⁴ Cayman’s COBRA1 Monoclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes COBRA1 at approximately 62 kDa from human, mouse, and rat samples.

WARNING This product is not for human or veterinary use.

1. Zhu, J., Song, S., Jiang, Z., et al. Characterization of COBRA1 in human breast cancer cell lines using a new polyclonal antibody against COBRA1. IUBMB Life 56(3), 161-166 (2004).

2. Aiyar, S.E., Cho, H., Lee, J., et al. Concerted transcriptional regulation by BRCA1 and COBRA1 in breast cancer cells. Int. J. Biol. Sci. 3(7), 486-492 (2007).

3. Sun, J., Blair, A.L., Aiyar, S.E., et al. Cofactor of BRCA1 modulates androgen-dependent transcription and alternative splicing. J. Steroid. Biochem. Mol. Biol. 107(3-5), 131-139 (2007).

4. Zhong, H., Zhu, J., Zhang, H., et al. COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun. 325(2), 568-573 (2004).