For immunochemical detection of connexin43
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Connexin43 Polyclonal Antibody

Item No. 29257

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • AVSD3
  • Cx43
  • Gap Junction Protein α1
  • GJAL
Immunogen
Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43
MW
~43 kDa
Formulation (Request formulation change)
100 µl of protein affinity-purified polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
WB
Species Reactivity
(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $374

    Add
    Cayman Chemical
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    Product Description

    Connexin43 (Cx43) is a membrane protein and member of the connexin family that is encoded by GJA1 in humans.1,2 It is widely expressed across various tissues, including the heart, brain, and retina, and exhibits plasma membrane localization. Cx43 is a monomeric component of connexons, homo- or heterohexamers that form hydrophilic pores and facilitate the exchange of ions and secondary messengers between the cytoplasm of connected cells at gap junctions. It is essential to cardiomyocyte function and cardiac development, osteoblast and osteocyte survival, and spermatogenesis.1 Cx43 is also expressed in various tumor cells, including in the liver, prostate, and breast, and exhibits tumor-specific pro- or anti-metastatic effects.1,3 Inhibition of Gja1 with antisense oligonucleotides promotes wound healing in murine skin, skeletal muscle, cardiac tissue, and vascular endothelium, however, global heterozygous knockdown of Gja1 increases infarct size in a mouse model of ischemia and reperfusion injury.1,2 Levels of Cx43 are decreased in postmortem retinal samples from patients with diabetes compared with control individuals.4 Cayman's Connexin43 Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes connexin43 at approximately 43 kDa from rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Prakoura, N., Kavvadas, P., and Chadjichristos, C.E. Connexin 43: A new therapeutic target against chronic kidney disease? Cell. Physiol. Biochem. 49(3), 998-1009 (2018).

    2. Schulz, R., Görge, P.M., Görbe, A., et al. Connexin 43 is an emerging therapeutic target in ischemia/reperfusion injury, cardioprotection and neuroprotection. Pharmacol. Ther. 153, 90-106 (2015).

    3. Li, Z., Zhou, Z., Welch, D.R., et al. Expressing connexin 43 in breast cancer cells reduces their metastasis to lungs. Clin. Exp. Metastasis 25(8), 893-901 (2008).

    4. Tien, T., Muto, T., Zhang, J., et al. Association of reduced connexin 43 expression with retinal vascular lesions in human diabetic retinopathy. Exp. Eye Res. 146, 103-106 (2016).