For immunochemical detection of connexin43 (phospho-Ser368)
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Connexin43 (Phospho-Ser368) Polyclonal Antibody

Item No. 29258

Product Insert (PDF)
Technical Information
Synonyms
  • Cx43
  • Gap Junction Protein α1
  • GJA1
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat connexin43
MW
~43 kDa
Formulation (Request formulation change)
100 µl of affinity-purified polyclonal antibody from pooled serum
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
WB
Species Reactivity
(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $341

    Add
    Cayman Chemical
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    Product Description

    Connexin43 (Cx43) is a membrane protein and member of the connexin family that is encoded by GJA1 in humans.1,2 It is widely expressed across various tissues, including heart, brain, and retina, and exhibits plasma membrane localization. Cx43 is a monomeric component of connexons, homo- or heterohexamers that form hydrophilic pores and facilitate the exchange of ions and secondary messengers between the cytoplasm of connected cells at gap junctions. It is essential to cardiomyocyte function and cardiac development, osteoblast and osteocyte survival, and spermatogenesis.1 Cx43 is also expressed in various tumor cells, including liver, prostate, and breast, and exhibits tumor-specific pro- or anti-metastatic effects.1,3 The C-terminus of Cx43 is subject to various post-translational modifications, including phosphorylation. Phosphorylation of Cx43 at position 386 (phospho-Ser368) is mediated by protein kinase C (PKC) and p38 MAPK.4,5 PKC-mediated phosphorylation of Cx43 is increased in a mouse model of acute respiratory distress syndrome (ARDS).4 IL-1β increases p38 MAPK-mediated phosphorylation of Cx43 at Ser368 in a perfused rat heart model of myocarditis.5 Cayman's Connexin43 (Phospho-Ser368) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes connexin43 (phospho-Ser368) at approximately 43 kDa from mouse and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Prakoura, N., Kavvadas, P., and Chadjichristos, C.E. Connexin 43: A new therapeutic target against chronic kidney disease? Cell. Physiol. Biochem. 49(3), 998-1009 (2018).

    2. Schulz, R., Görge, P.M., Görbe, A., et al. Connexin 43 is an emerging therapeutic target in ischemia/reperfusion injury, cardioprotection and neuroprotection. Pharmacol. Ther. 153, 90-106 (2015).

    3. Li, Z., Zhou, Z., Welch, D.R., et al. Expressing connexin 43 in breast cancer cells reduces their metastasis to lungs. Clin. Exp. Metastasis 25(8), 893-901 (2008).

    4. Liu, T., Li, Y., Zhang, B., et al. The role of phosphorylated Cx43 on PKC mediated Ser368 in lung injury induced by seawater inhalation. Inflammation 38(5), 1847-1854 (2015).

    5. Zhong, Z., Chang, H., Wu, Y., et al. Up-regulated Cx43 phosphorylation at Ser368 prolongs QRS duration in myocarditis. J. Cell Mol. Med. 22(7), 3537-3547 (2017).