For immunochemical detection of NeuN
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NeuN Monoclonal Antibody (Clone 1B7)

Item No. 29265

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • FOX3
  • Rbfox3
  • RNA Binding Protein Fox-1 Homolog 3
Immunogen
Recombinant protein from the N-terminus of human NeuN
Clone Designation
1B7
MW
46 and 48 kDa for nuclear and cytosolic NeuN, respectively
Formulation (Request formulation change)
100 µl of Protein G-purified mouse monoclonal antibody
Storage Buffer
PBS with 50% glycerol and 5 mM sodium azide
Host
Mouse
Isotype
IgG2a
Applications
ICC, IHC, and WB
Species Reactivity
(+) Human(+) Bovine(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $376

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    Product Description

    NeuN, also known as FOX3 and RNA binding protein fox-1 homolog 3 (Rbfox3), is a pre-mRNA alternative splicing regulator encoded by RBFOX3 in humans.1,2 It is expressed in mature neurons of the brain and spinal cord and is commonly used as a neuronal marker to quantify the number of new neurons generated during adult neurogenesis or the extent of therapeutic neuroprotection in animal models of neurodegenerative disease.3,4 There are two subtypes of NeuN, a 46 kDa nuclear form and 48 kDa cytoplasmic form. Cytosolic NeuN is increased in the lumbar spinal cord in a mouse model of amyotrophic lateral sclerosis (ALS) compared with control animals.2 Exon deletions and truncations of NeuN are found in patients with Rolandic epilepsy and RBFOX3 is located within the apparently balanced chromosomal rearrangement (ABCR) regions of chromosomes in patients with developmental delays and speech disorders.4 Cayman's NeuN Monoclonal Antibody (Clone 1B7) can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes nuclear and cytosolic NeuN at approximately 46 and 48 kDa, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kim, K.K., Adelstein, R.S., and Kawamoto, S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. The Journal of Biological Chemisty 284(45), 31052-31061 (2009).

    2. Ma, X., Turnbull, P.C., Crapper, E.P., et al. Cytosolic localization of Fox proteins in motor neurons of G93A SOD1 mice. Histochem. Cell Biol. 145(5), 545-559 (2016).

    3. Kempermann, G., and Gage, F.H. Genetic influence on phenotypic differentiation in adult hippocampal neurogenesis. Brain Res. Dev. Brain Res. 134(1-2), 1-12 (2002).

    4. Duan, W., Zhang, Y.-P., Hou, Z., et al. Novel insights into NeuN: From neuronal marker to splicing regulator. Mol. Neurobiol. 53(3), 1637-1647 (2016).