For immunochemical detection of the GABAA receptor α1 subunit
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GABAA Receptor α1 Subunit (N-Term) Polyclonal Antibody

Item No. 29267

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • γ-Aminobutyric Acid Receptor Subunit α1
  • GABAA Receptor Subunit α1
  • GABRA1
Immunogen
Peptide from the N-terminal region of the α1 subunit of the rat GABAA receptor conjugated to keyhole limpet hemocyanin (KLH)
MW
~51 kDa
Formulation (Request formulation change)
100 µl of Affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
IHC, and WB
Species Reactivity
(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $510

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    Product Description

    GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.1,2 They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: α1-6, β1-4, γ1-3, δ, ε, π, θ, and ρ1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by α1β2γ2 subunit-containing GABAA receptors, the major isoform found in the brain.2,3 The α subunit of GABAA receptors interfaces with a β subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the γ subunit. Mutations in GABRA1, which encodes the α1 subunit isoform, are found in patients with autosomal dominant juvenile myoclonic epilepsy (JME), infantile spasms, and childhood absence epilepsy (CAE).2 GABRA1 expression is upregulated in ipsilateral, but not contralateral, perilesional tissue in a rat model of cortical ischemia-reperfusion injury.4 Cayman’s GABAA Receptor α1 Subunit (N-Term) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor α1 subunit at approximately 51 kDa from mouse and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Crestani, F., and Rudolph, U. Behavioral functions of GABAA receptor subtypes - the Zurich experience. Diversity and Functions of GABA receptors: A tribute to Hanns Möhler, part A 37-51 (2015).

    2. Hirose, S. Mutant GABAA receptor subunits in genetic (idiopathic) epilepsy. Genetics of epilepsy 55-85 (2014).

    3. Wongsamitkul, N., Maldifassi, M.C., Simeone, X., et al. α subunits in GABAA receptors are dispensable for GABA and diazepam action. Sci. Rep. 7(1), 15498 (2017).

    4. Neumann-Haefelin, T., Bosse, F., Redecker, C., et al. Upregulation of GABAA-receptor α1- and α2-subunit mRNAs following ischemic cortical lesions in rats. Brain Res. 816(1), 234-237 (1999).