For immunochemical detection of the GABAA receptor α4 subunit
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GABAA Receptor α4 Subunit Polyclonal Antibody

Item No. 29270

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Gamma-aminobutyric Acid (GABA) A Receptor, α4
  • Gamma-aminobutyric Acid Receptor Subunit α4
  • GABAA Receptor Subunit α4
Immunogen
Fusion protein from the cytoplasmic loop of the α4 subunit of the rat GABAA receptor
MW
~64 kDa
Formulation (Request formulation change)
100 µl of affinity-purified pooled rabbit serum polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
IHC, WB
Species Reactivity
(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $534

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    Product Description

    GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.1,2 They are postsynaptic heteropentameric receptors that contains protein subunits from the following isoforms: α1-6, β1-4, γ1-3, δ, ε, π, θ, and ρ1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by α1β2γ2 subunit-containing GABAA receptors, the major isoform found in the brain.2,3 The α subunit of GABAA receptors interfaces with a β subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the γ subunit. The GABAA receptor α4 subunit is found at high levels in the thalamus and hippocampal dentate gyrus, with moderate levels in the cortex and low levels in other brain regions.4 Knockdown of Gabrar4, which encodes the α4 subunit isoform, inhibits tonic inhibition in thalamic relay neurons and prevents sedative effects induced by the GABAA receptor agonist gaboxadol (Item No. 16355) or ethanol in mice. Gabrar4 knockout also inhibits the development of tolerance in a rat model of chronic intermittent ethanol administration.5 Cayman's GABAA Receptor α4 Subunit Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor α4 subunit at approximately 64 kDa from mouse and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Crestani, F., and Rudolph, U. Behavioral functions of GABAA receptor subtypes - the Zurich experience. Diversity and Functions of GABA receptors: A tribute to Hanns Möhler, part A 37-51 (2015).

    2. Hirose, S. Mutant GABAA receptor subunits in genetic (idiopathic) epilepsy. Genetics of epilepsy 55-85 (2014).

    3. Wongsamitkul, N., Maldifassi, M.C., Simeone, X., et al. α subunits in GABAA receptors are dispensable for GABA and diazepam action. Sci. Rep. 7(1), 15498 (2017).

    4. Jia, F., Pignataro, L., and Harrison, N.L. GABAA receptors in the thalamus: α4 subunit expression and alcohol sensitivity. Alcohol 41(3), 177-185 (2007).

    5. Olsen, R.W., and Liang, J. Role of GABAA receptors in alcohol use disorders suggested by chronic intermittent ehtanol (CIE) rodent model. Mol. Brain 10(1), 45 (2017).