For immunochemical detection of the GABAA receptor α5 subunit
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GABAA Receptor α5 Subunit Polyclonal Antibody

Item No. 29271

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • γ-Aminobutyric Acid Receptor Subunit α5
  • GABAA Receptor Subunit α5
  • GABRA5
Immunogen
Fusion protein from the cytoplasmic loop of the α5 subunit of the rat GABAA receptor
MW
~55 kDa
Formulation (Request formulation change)
100 µl of Affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
IHC and WB
Species Reactivity
(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $510

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    Product Description

    GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.1,2 They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: α1-6, β1-4, γ1-3, δ, ε, π, θ, and ρ1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by α1β2γ2 subunit-containing GABAA receptors, the major isoform found in the brain.2,3 The α subunit of GABAA receptors interfaces with a β subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the γ subunit. Approximately 5% of all GABAA receptors contain α5 subunits and up to 25% of these receptors are expressed in the hippocampus, a region of the brain associated with learning and memory.4 Deletions in GABRA5, which encodes the α5 subunit isoform, have been found on chromosome 15 in patients with Angelman syndrome who typically develop autism spectrum disorder (ASD). Levels of α5 subunit-containing GABAA receptors are decreased in postmortem hippocampal samples from patients with advanced Alzheimer’s disease, Down syndrome, or fetal alcohol syndrome. Cayman’s GABAA Receptor α5 Subunit Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor α5 subunit at approximately 55 kDa from mouse and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Crestani, F., and Rudolph, U. Behavioral functions of GABAA receptor subtypes - the Zurich experience. Diversity and Functions of GABA receptors: A tribute to Hanns Möhler, part A 37-51 (2015).

    2. Hirose, S. Mutant GABAA receptor subunits in genetic (idiopathic) epilepsy. Genetics of epilepsy 55-85 (2014).

    3. Wongsamitkul, N., Maldifassi, M.C., Simeone, X., et al. α subunits in GABAA receptors are dispensable for GABA and diazepam action. Sci. Rep. 7(1), 15498 (2017).

    4. Mohamad, F.H., and Has, A.T.C. The α5-containing GABAA receptors-a brief summary. J. Mol. Neurosci. 67(2), 343-351 (2019).