For immunochemical detection of the GABAA receptor α6 subunit
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GABAA Receptor α6 Subunit Polyclonal Antibody

Item No. 29272

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • γ-Aminobutyric Acid Receptor Subunit α6
  • γ-Aminobutyric Acid (GABA) A Receptor, α6
  • GABAA Receptor Subunit α6
Immunogen
Fusion protein from the cytoplasmic loop of the α6 subunit of the rat GABAA receptor
MW
~57 kDa
Formulation (Request formulation change)
100 µl of affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg BSA per ml, and 50% glycerol
Host
Rabbit
Applications
WB
Species Reactivity
(+) Mouse(+) rat; other species not tested
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $534

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    Product Description

    GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.1,2 They are postsynaptic heteropentameric receptors that contains protein subunits from the following isoforms: α1-6, β1-4, γ1-3, δ, ε, π, θ, and ρ1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by α1β2γ2 subunit-containing GABAA receptors, the major isoform found in the brain.2,3 The α subunit of GABAA receptors interfaces with a β subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the γ subunit. The GABAA receptor α6 subunit is expressed in granule cells of the cerebellum and cochlear nucleus.4 Expression of GABRA6, which encodes the α6 subunit isoform, is decreased in withdrawal seizure-prone, but not withdrawal seizure-resistant, mice after chronic ethanol administration. Point mutation of the arginine residue at position 100 (R100Q) of the GABAA receptor α6 subunit increases sensitivity to diazepam and ethanol in rats and humans.5 Cayman’s GABAA Receptor α6 Subunit Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes the GABAA receptor α6 subunit at approximately 57 kDa from mouse and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Crestani, F., and Rudolph, U. Behavioral functions of GABAA receptor subtypes - the Zurich experience. Diversity and Functions of GABA receptors: A tribute to Hanns Möhler, part A 37-51 (2015).

    2. Hirose, S. Mutant GABAA receptor subunits in genetic (idiopathic) epilepsy. Genetics of epilepsy 55-85 (2014).

    3. Wongsamitkul, N., Maldifassi, M.C., Simeone, X., et al. α subunits in GABAA receptors are dispensable for GABA and diazepam action. Sci. Rep. 7(1), 15498 (2017).

    4. Loh, E.-W., and Ball, D. Role of the GABAAβ2, GABAAα6, GABAAα1 and GABAAγ2 receptor subunit genes cluster in drug responses and the development of alcohol dependence. Neurochem. Int. 37(5-6), 413-423 (2000).

    5. Sanna, A., Congeddu, E., Saba, L., et al. The cerebellar GABAA α6 subunit is differentially modulated by chronic ethanol exposure in normal (R100R) and mutated (Q100Q) sNP rats. Brain Res. 998(2), 148-154 (2004).