For immunochemical detection of the GABAA receptor β3 subunit
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GABAA Receptor β3 Subunit Polyclonal Antibody

Item No. 29275

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GABRB3
  • Gamma-aminobutyric Acid Receptor Subunit β3
Immunogen
Fusion protein from the cytoplasmic loop of the β3 subunit of the rat GABAA receptor
MW
~53 kDa
Formulation (Request formulation change)
100 µl of Affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
IHC, WB
Species Reactivity
(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $528

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    Product Description

    GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.1,2 They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: α1-6, β1-4, γ1-3, δ, ε, π, θ, and ρ1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by α1β2γ2 subunit-containing GABAA receptors, the major isoform found in the brain.2,3 The β subunit of GABAA receptors interfaces with an α subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the γ subunit. β3 subunit-containing GABAA receptors are widely expressed in the cerebral cortex, cerebellum, olfactory bulb, and hippocampus.4 Phosphorylation of the β3 subunit by PKA or PKC inhibits binding of the β3 subunit with the clathrin adaptor protein AP2 and reduces GABAA receptor endocytosis.5 Mutations in GABRB3, which encodes the β3 subunit isoform, have been found in patients with childhood absence epilepsy (CAE), infantile spasms (IS), and Lennox-Gastaut syndrome (LGS).2 Cayman's GABAA Receptor β3 Subunit Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor β3 subunit at approximately 53 kDa from rat and mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Crestani, F., and Rudolph, U. Behavioral functions of GABAA receptor subtypes - the Zurich experience. Diversity and Functions of GABA receptors: A tribute to Hanns Möhler, part A 37-51 (2015).

    2. Hirose, S. Mutant GABAA receptor subunits in genetic (idiopathic) epilepsy. Genetics of epilepsy 55-85 (2014).

    3. Wongsamitkul, N., Maldifassi, M.C., Simeone, X., et al. α subunits in GABAA receptors are dispensable for GABA and diazepam action. Sci. Rep. 7(1), 15498 (2017).

    4. Miralles, C.P., Li, M., Mehta, A.K., et al. Immunocytochemical localization of the β3 subunit of the γ-aminobutyric acidA receptor in the rat brain. J. Comp. Neurol. 413(4), 535-548 (1999).

    5. Vithlani, M., Moss, S.J., and Terunuma, M. The dynamic modulation of GABAA receptor trafficking and its role in the formation of inhibitory synapses. Physiol. Rev. 91(3), 1009-1022 (2011).