GluR1 Monoclonal Antibody (Clone RH95)

AMPA receptors are ionotropic glutamate receptors that mediate excitatory synaptic transmission.^1,2 They are tetrameric protein complexes expressed throughout the central nervous system in both neurons and glia that are assembled from combinations of GluR1, GluR2, GluR3, and GluR4, also known as GluR-A-D, subunits, each of which has extracellular N-terminal and ligand binding domains, a channel domain consisting of three membrane-spanning helices and a channel pore loop, and an intracellular C-terminus.^2,3,4,5 The GluR1 subunit, which is encoded by GRIA1 in humans, is primarily expressed in the forebrain and hippocampus. Mice lacking the GluR1 subunit exhibit normal spatial reference memory but have deficits in spatial working memory.¹ They also exhibit increased and decreased duration of nocifensive behaviors in phases I and II, respectively, of the formalin test, as well as increased duration of nocifensive behaviors in response to intraplantar injection of capsaicin (Item No. 92350), compared with wild-type mice.⁶ SNPs in GRIA1 have been found in patients with bipolar disorder with psychotic features.⁴ Elevated levels of GRIA1 mRNA have been found in postmortem dorsolateral prefrontal cortex from patients with schizophrenia.⁷ Cayman's GluR1 Monoclonal Antibody (Clone RH95) can be used for Western blot (WB) applications. The antibody recognizes GluR1 at approximately 105 kDa from mouse and rat samples.

WARNING This product is not for human or veterinary use.

1. Sanderson, D.J., Good, M.A., Seeburg, P.H., et al. The role of the GluR-A (GluR1) AMPA receptor subunit in learning and memory. Progress in Brain Research: Essence of Memory 169, 159-178 (2008).

2. Isaac, J.T.R., Ashby, M.C., and McBain, C.J. The role of the GluR2 subunit in AMPA receptor function and synaptic plasticity. Neuron 54(6), 859-871 (2007).

3. Bassani, S., Valnegri, P., Beretta, F., et al. The GLUR2 subunit of AMPA receptors: Synaptic role. Neuroscience 158(1), 55-61 (2009).

4. Kerner, B., Jasinska, A.J., DeYoung, J., et al. Polymorphisms in the GRIA1 gene region in psychotic bipolar disorder. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B(1), 24-32 (2009).

5. Sprengel, R. Role of AMPA receptors in synaptic plasticity. Cell Tissue Res. 326(2), 447-455 (2006).

6. Hartmann, B., Ahmadi, S., Heppenstall, P.A., et al. The AMPA receptor subunits GluR-A and GluR-B reciprocally modulate spinal synaptic plasticity and inflammatory pain. Neuron 44(4), 637-650 (2004).

7. O'Connor, J.A., and Hemby, S.E. Elevated GRIA1 mRNA expression in layer II/III and V pyramidal cells of the DLPFC in schizophrenia. Schizophr. Res. 97(1-3), 277-288 (2007).