Mitochondrial Fission Factor Polyclonal Antibody

Mitochondrial fission factor (MFF) is a tail-anchored membrane protein with roles in mitochondrial and peroxisomal fission.^1,2 It is localized to the outer membrane of mitochondria, as well as the membrane-restricted regions of elongated peroxisomes. It is composed of a cytoplasmic N-terminus that functions as a receptor for the GTPases dynamin-related protein 1 (DRP1) and dynamin-like protein 1 (DLP1), a central coiled-coil domain, and a C-terminal transmembrane domain.³ siRNA knockdown of MFF inhibits DRP1 recruitment and mitochondrial fission induced by loss of mitochondrial membrane potential and delays cytochrome c release from mitochondria and apoptotic progression in vitro.^1,3 MicroRNA knockdown of MFF inhibits norepinephrine-induced hypertrophy and mitochondrial fission in isolated neonatal rat ventricular cells.³ MFF is overexpressed in various cancer cells and knockdown of MFF reduces tumor volume in a PC3 mouse xenograft model.⁴ Cayman's Mitochondrial Fission Factor Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes MFF at approximately 38 kDa from human, bovine, and mouse samples.

WARNING This product is not for human or veterinary use.

1. Gandre-Babbe, S., and van der Bliek, A.M. The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Mol. Biol. Cell 19(6), 2402-2412 (2008).

2. Itoyama, A., Michiyuki, S., Honsho, M., et al. Mff functions with Pex11pβ and DLP1 in peroxisomal fission. Biol. Open 2(10), 998-1006 (2013).

3. Sun, Y.L., Li, S.H., Yang, L., et al. miR-376b-3p attenuates mitochondrial fission and cardiac hypertrophy by targeting mitochondrial fission factor. Clin. Exp. Pharmacol. Physiol. 45(8), 779-787 (2018).

4. Seo, J.H., Agarwal, E., Chae, Y.C., et al. Mitochondrial fission factor is a novel Myc-dependent regulator of mitochondrial permeability in cancer. EBioMedicine 48, 353-363 (2019).