For immunochemical detection of NCC (Phospho-Thr53)
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NCC (Phospho-Thr53) Polyclonal Antibody

Item No. 29285

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Sodium Chloride Symporter
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr53 of mouse NCC
MW
~160 kDa
Formulation (Request formulation change)
100 µl of affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg BSA per ml, and 50% glycerol
Host
Rabbit
Applications
IF and WB
Cross Reactivity
(+) NCC (phospho-Thr53)(-) Unphosphorylated NCC
Species Reactivity
(+) Human(+) mouse(+) rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $534

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    Product Description

    The thiazide-sensitive sodium chloride cotransporter (NCC) is a member of the SLC12 family of transporters and is encoded by SLC12A3 in humans.1 NCC is expressed in epithelial cells of the distal convoluted tubule and localizes to the apical plasma membrane where it mediates sodium reabsorption in the kidney. It consists of a central hydrophobic domain with 12 transmembrane helices that contain affinity-modifying residues for sodium, chloride, and thiazides that is flanked by intracellular N- and C-terminal domains with sites that are subject to phosphorylation. Phosphorylation of NCC at threonine 53 (Thr53) is mediated by serum- and glucocorticoid-inducible kinase 1 (SGK1), STE20/SPS1-related proline-alanine-rich protein kinase (SPAK), or oxidative stress-responsive kinase 1 (OSR1). X. laevis oocytes expressing a threonine-to-alanine substitution at Thr53 in NCC, which abolishes its phosphorylation, have reduced chloride deprivation-induced sodium uptake, but not NCC surface expression, compared to wild-type oocytes.2 NCC (phospho-Thr53) levels are increased in the kidney by low dietary intake of sodium chloride or potassium in mice.3 NCC (phospho-Thr53) levels are also increased in the kidney of the CUL3-Het/∆9 mouse model of familial hyperkalemic hypertension.4 Cayman's NCC (Phospho-Thr53) Polyclonal Antibody can be used for immunofluorescence (IF) and Western blot (WB) applications. The antibody recognizes NCC (phospho-Thr53) at approximately 160 kDa from human, mouse, and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Moes, A.D., van der Lubbe, N., Zietse, R., et al. The sodium chloride cotransporter SLC12A3: New roles in sodium, potassium, and blood pressure regulation. Pflugers Arch. 466(1), 107-118 (2014).

    2. Pacheco-Alvarez, D., Cristóbal, P.S., Meade, P., et al. The Na+:Cl- cotransporter is activated and phosphorylated at the amino-terminal domain upon intracellular chloride depletion. The Journal of Biological Chemisty 281(39), 28755-28763 (2006).

    3. Vallon, V., Schroth, J., Lang, F., et al. Expression and phosphorylation of the Na+-Cl- cotransporter NCC in vivo is regulated by dietary salt, potassium, and SGK1. Am. J. Physiol. Renal Physiol. 297(3), F704-F712 (2009).

    4. Ferdaus, M.Z., Miller, L.N., Agbor, L.N., et al. Mutant cullin 3 causes familial hyperkalemic hypertension via dominant effects. JCI Insight 2(24), e96700 (2017).