Nestin Monoclonal Antibody (Clone 4D11)

Nestin, also known as neuroepithelial stem cell protein, is a type VI intermediate filament protein.¹ Nestin monomers are comprised of a short N-terminal head domain that is required for assembly of intermediate filaments, an α-helical rod domain that mediates dimerization, and a long C-terminal tail.¹ They form heterodimers and heterotetramers with other intermediate filament proteins, including vimentin and α-internexin, for assembly into intermediate filaments.^1,2 Nestin is found in a variety of tissue types but is expressed primarily in nervous tissue during embryonic development in mammals and has been used as a marker of neural stem/progenitor cells (NSPCs).^2,3 Nestin synthesis decreases as nervous tissues differentiate, however, nestin-positive neural stem cells are found in the subventricular zone (SVZ) and hippocampal dentate gyrus in the adult mammalian brain. Nestin is also expressed in a variety of tumor types and cancer stem cells, as well as in cerebellar Purkinje cells from patients with Creutzfeldt-Jakob disease (CJD).^3,4 Cayman’s Nestin Monoclonal Antibody (Clone 4D11) can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes a nestin doublet at approximately 220 to 240 kDa from human, mouse, and rat samples.

WARNING This product is not for human or veterinary use.

1. Bernal, A., and Arranz, L. Nesti-expressing progenitor cells: Function, identity and therapeutic implications. Cell. Mol. Life Sci. 75(12), 2177-2195 (2018).

2. Gilyarov, A.V. Nestin in central nervous system cells. Neurosci. Behav. Physiol. 38(2), 165-169 (2008).

3. Krupkova, O., Jr., Loja, T., Zambo, I., et al. Nestin expression in human tumors and tumor cell lines. Neoplasma 57(4), 291-298 (2010).

4. Mizuno, Y., Takeuchi, T., Takatama, M., et al. Expression of nestin in Purkinje cells in patients with Creutzfeldt-Jakob disease. Neurosci. Lett. 352(2), 109-112 (2003).