For immunochemical detection of KCC2 (Phospho-Ser940)
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KCC2 (Phospho-Ser940) Polyclonal Antibody

Item No. 29291

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Electroneutral Potassium Chloride Cotransporter 2
  • Neuronal Potassium Chloride Cotransporter
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser940 of rat KCC2
MW
~135 kDa
Formulation (Request formulation change)
100 µl of affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Applications
WB
Cross Reactivity
(+) KCC2 (phospho-Ser940)(-) Non-phosphorylated KCC2
Species Reactivity
(+) Mouse(+) rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $436

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    Product Description

    The neuronal-specific potassium chloride cotransporter 2 (KCC2) is a member of the SLC12 family of transporters and is encoded by SLC12A5 in humans.1 KCC2 is expressed in mature neurons throughout the CNS and localizes to the soma and dendrite plasma membrane where it mediates chloride ion efflux, maintaining the transmembrane chloride potential.2 It consists of 12 transmembrane helices, which contain an extracellular loop subject to N-glycosylation, as well as intracellular N- and C-terminal domains with sites that are subject to phosphorylation. Phosphorylation of KCC2 at serine 940 (Ser940) is mediated by PKC and regulates KCC2 stability and expression.1,3 KCC2 (phospho-Ser940) levels are increased by the PKC activator phorbol 12-myristate 13-acetate (PMA; Item No. 10008014) in primary rat embryonic hippocampal neurons.3 HEK293 cells expressing a point mutation of Ser940 (S940A) in KCC2, which abolishes its phosphorylation, have a reduced rate of ion transport and decreased KCC2 endocytosis. Mice expressing S940A have reduced latency to first seizure and increased mortality in a mouse model of status epilepticus induced by kainate.4 Cayman's KCC2 (Phospho-Ser940) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes KCC2 (Phospho-Ser940) at approximately 135 kDa from mouse and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Côme, E., Heubl, M., Schwartz, E.J., et al. Reciprocal regulation of KCC2 trafficking and synaptic activity. Front. Cell Neurosci. 13, 48 (2019).

    2. Chamma, I., Chevy, Q., Poncer, J.C., et al. Role of the neuronal K-Cl co-transporter KCC2 in inhibitory and excitatory neurotransmission. Front. Cell Neurosci. 6, 5 (2012).

    3. Lee, H.H.C., Walker, J.A., Williams, J.R., et al. Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2. The Journal of Biological Chemisty 282(41), 29777-29784 (2007).

    4. Silayeva, L., Deeb, T.Z., Hines, R.M., et al. KCC2 activity is critical in limiting the onset and severity of status epilepticus. PNAS 112(11), 3523-3528 (2015).