KCC2 (Phospho-Thr¹⁰⁰⁷) Polyclonal Antibody

The neuronal-specific potassium chloride cotransporter 2 (KCC2) is a member of the SLC12 family of transporters and is encoded by SLC12A5 in humans.¹ KCC2 is expressed in mature neurons throughout the CNS and localizes to the soma and dendrite plasma membrane where it mediates chloride ion efflux, maintaining the transmembrane chloride potential.² It consists of 12 transmembrane helices, which contain an extracellular loop subject to N-glycosylation, as well as intracellular N- and C-terminal domains with sites that are subject to phosphorylation. Phosphorylation of KCC2 at threonine 1007 (Thr¹⁰⁰⁷) is mediated by lysine-deficient protein kinase 1 (WNK1) and its effectors, STE20/SPS1-related proline-alanine-rich protein kinase (SPAK) and oxidative stress-responsive kinase 1 (OSR1), resulting in reduced KCC2 activity. Phospho-Thr1007 levels are decreased by the KCC2 activator N-ethylmaleimide (NEM) in HEK293 cells.³ Point mutation of Thr¹⁰⁰⁷ (T1007E) in KCC2 mimics its phosphorylation and abolishes NEM-induced chloride ion efflux in HEK293 cells. Cayman's KCC2 (Phospho-Thr¹⁰⁰⁷) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes KCC2 (phospho-Thr¹⁰⁰⁷) at approximately 135 kDa from mouse and rat samples.

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1. Côme, E., Heubl, M., Schwartz, E.J., et al. Reciprocal regulation of KCC2 trafficking and synaptic activity. Front. Cell Neurosci. 13, 48 (2019).

2. Chamma, I., Chevy, Q., Poncer, J.C., et al. Role of the neuronal K^-Cl co-transporter KCC2 in inhibitory and excitatory neurotransmission. Front. Cell Neurosci. 6, 5 (2012).

3. Conway, L.C., Cardarelli, R.A., Moore, Y.E., et al. N-Ethylmaleimide increases KCC2 cotransporter activity by modulating transporter phosphorylation. The Journal of Biological Chemisty 292(52), 21253-21263 (2017).