For immunochemical detection of native tyrosine hydroxylase
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Tyrosine Hydroxylase (rat, native) Polyclonal Antibody

Item No. 29299

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • TH
  • Tyrosine 3-hydroxylase
  • Tyrosine 3-monooxygenase
Immunogen
Native rat tyrosine hydroxylase purified from pheochromocytoma
MW
~60 kDa
Formulation (Request formulation change)
100 µl affinity-purified polyclonal antibody from pooled serum
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Sheep
Applications
ICC, IHC, WB
Species Reactivity
(+) Human(+) Mouse(+) Rabbit(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $516

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    Product Description

    Tyrosine hydroxylase (TH) catalyzes the conversion of tyrosine to L-DOPA, which is the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine.1 It assembles into tetramers, with each monomer comprised of an N-terminal regulatory domain with three serine residues at positions 19, 31, and 40 that are subject to regulatory phosphorylation, a central catalytic domain, and a C-terminal tetramerization domain. TH is expressed in dopaminergic neurons in the olfactory bulb, diencephalon, substantia nigra, ventral tegmental area, and retinal amacrine cells, adrenergic and noradrenergic cells in the hypothalamus, medulla, and locus coeruleus (LC), as well as sympathetic ganglia and adrenal chromaffin cells. Due to its expression in catecholaminergic neurons, TH has been used as a marker of these cells in the brain.2 Mutations in TH have been found in patients with tyrosine hydroxylase deficiency, infantile parkinsonism, and progressive infantile encephalopathy.3,4 Cayman's Tyrosine Hydroxylase (rat, native) Polyclonal Antibody can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes TH at approximately 60 kDa from human, mouse, rabbit, and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tekin, I., Roskoski, R., Jr., Carkaci-Salli, N., et al. Complex molecular regulation of tyrosine hydroxylase. J. Neural Transm. (Vienna) 121(12), 1451-1481 (2014).

    2. Berod, A., Hartman, B.K., Keller, A., et al. A new double labeling technique using tyrosine hydroxylase and dopamine-β-hydroxylase immunohistochemistry: Evidence for dopaminergic cells lying in the pons of the beef brain. Brain Res. 240(2), 235-243 (1982).

    3. Willemsen, M.A., Verbeek, M.M., Kamsteeg, E.-J., et al. Tyrosine hydroxylase deficiency: A treatable disorder of brain catecholamine biosynthesis. Brain 133(Pt. 6), 1810-1822 (2010).

    4. White, R.B., and Thomas, M.G. Moving beyond tyrosine hydroxylase to define dopaminergic neurons for use in cell replacement therapies for Parkinson’s disease. CNS Neurol. Disord. Drug Targets 11(4), 340-349 (2012).