A colorimetric β-galactosidase substrate
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Chlorophenol Red β-D-Galactopyranoside (sodium salt)

Item No. 29707

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-chloro-4-[3-(3-chloro-4-hydroxyphenyl)-1,1-dioxido-3H-2,1-benzoxathiol-3-yl]phenyl β-D-galactopyranoside, monosodium salt
Synonyms
  • CPRG
Molecular Formula
C25H22Cl2O10S • Na
Formula Weight
608.4
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
270, 415 nm
SMILES
OC[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@H](OC2=C(Cl)C=C(C(O3)(C4=CC(Cl)=C(O)C=C4)C5=C(C=CC=C5)S3(=O)=O)C=C2)O1.[Na+]
InChi Code
InChI=1S/C25H22Cl2O10S.Na/c26-15-9-12(5-7-17(15)29)25(14-3-1-2-4-20(14)38(33,34)37-25)13-6-8-18(16(27)10-13)35-24-23(32)22(31)21(30)19(11-28)36-24;/h1-10,19,21-24,28-32H,11H2;/q;+1/t19-,21+,22+,23-,24-,25?;/m1./s1
InChi Key
NLWTWQKLKSPEKU-PMTFPNBHSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    50 mg

    $108

    Add

    100 mg

    $205

    Add

    250 mg

    $486

    Add

    500 mg

    $917

    Add
    Product Description

    Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate for β-galactosidase.1,2 Upon enzymatic cleavage by β-galactosidase, chlorophenol red is released, which can be quantified by colorimetric detection at 570 nm as a measure of β-galactosidase activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sicard, C., Shek, N., White, D., et al. A rapid and sensitive fluorimetric β-galactosidase assay for coliform detection using chlorophenol red-β-ᴅ-galactopyranoside. Anal. Bioanal. Chem. 406(22), 5395-5403 (2014).

    2. Jendresen, C., Daws, M.R., and Nilsson, L.N.G. An improved CPRG colorimetric ligand-receptor signal transduction assay based on beta-galactosidase activity in mammalian BWZ-reporter cells. J. Pharmacol. Toxicol. Methods 90, 67-75 (2018).