Scavenger Receptor B2/CD36 Blocking Peptide

To be used in conjunction with Cayman’s CD36 polyclonal antibody (Item No. 100011) to block protein-antibody complex formation during immunochemical analysis of CD36. CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells in atherosclerotic lesions.^1,2,3,4 This membrane glycoprotein can internalize fatty acids which activate PPARγ and stimulate further expression of CD36.^5,6 This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments, and post-apoptotic monocytes and neutrophils.^7,8,9,10 CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.¹¹ This transmembrane glycoprotein is detected by immunoblotting at 88 kDa or 54 kDa depending on the degree of glycosylation of the receptor in your sample. Positive controls include adipose and heart tissue, platelets, and macrophages.

WARNING This product is not for human or veterinary use.

1. Febbraio, M., Abumrad, N.A., Hajjar, D.P., et al. A null mutation in murine CD36 reveals an important role in fatty acid and lipoprotein metabolism. The Journal of Biological Chemisty 274(27), 19055-19062 (1999).

2. Podrez, E.A., Febbraio, M., Sheibani, N., et al. Macrophage scavenger receptor CD36 is the major receptor for LDL modified by monocyte-generated reactive nitrogen species. J. Clin. Invest. 105(8), 1095-1108 (2000).

3. Febbraio, M., Hajjar, D.P., and Silverstein, R.L. CD36: A class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. J. Clin. Invest. 108(6), 785-791 (2001).

4. Han, J., Hajjar, D.P., Febbraio, M., et al. Native and modified low density lipoproteins increase the functional expression of the macrophage class B scavenger receptor, CD36. The Journal of Biological Chemisty 272(34), 21654-21659 (1997).

5. Tontonoz, P., Nagy, L., Alvarez, J.G.A., et al. PPARγ promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell 93(2), 241-252 (1998).

6. Greenwalt, D.E., Lipsky, R.H., Ockenhouse, C.F., et al. Membrane glycoprotein CD36: A review of its roles in adherence, signal transduction, and transfusion medicine. Blood 80(5), 1105-1115 (1992).

7. Finnemann, S.C., and Silverstein, R.L. Differential roles of CD36 and αvβ5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium. J. Exp. Med. 194(9), 1289-1298 (2001).

8. Navazo, M.D.P., Daviet, L., Savill, J., et al. Identification of a domain (155-183) on CD36 implicated in the phagocytosis of apoptotic neutrophils. The Journal of Biological Chemisty 271(26), 15381-15385 (1996).

9. Jiménez, B., Volpert, O.V., Crawford, S.E., et al. Signals leading to apoptosis-dependent inhibition of neovascularization by thrombospondin-1. Nat. Med. 6(1), 41-48 (2000).

10. Rutella, S., Rumi, C., Puggioni, P., et al. Expression of thrombospondin receptor (CD36) in B-cell chronic lymphocytic leukemia as an indicator of tumor cell dissemination. Haematologica 84(5), 419-424 (1999).