Citrullinated Histone H2B (human, recombinant)

Citrullinated Histone H2B Type 1-A is a citrullinated form of human histone H2B type 1-A (hTSH2B), a testis- and sperm-specific variant of the histone H2B protein that is encoded by H2BC1, previously known as HIST1H2BA.^1,2 hTSH2B shares 85% identity with human somatic H2B.1, with the majority of amino acid differences between hTSH2B and somatic H2B occurring at the N-terminal end of the protein.¹ H2BC1 is expressed in testis, and protein levels of hTSH2B are elevated in early round spermatids, decreasing over the course of spermiogenesis, with hTSH2B present in approximately 20% of mature spermatozoa.^1,3 hTSH2B is enriched at promoter regions of genes important for sperm biology, capacitation, and fertilization and localizes to the basal nuclear area in mature spermatozoa.^1,4 When incubated in Xenopus egg extracts, hTSH2B-positive human sperm cells decondense more rapidly and have increased long nuclear axis lengths compared with hTSH2B-negative sperm cells.⁵ Mice lacking the genes encoding the murine homolog of hTSH2B (TH2B), as well as the histone variant TH2A (Th2a^-/-Th2b^-/- mice), are sterile and have spermatogenesis defects.⁶ This product contains purified histone H2B type 1-A (human, recombinant) (Item No. 11081) that has been modified with PAD4 enzyme, which is subsequently depleted by affinity chromatography. Cayman's Citrullinated Histone H2B Type 1-A (human, recombinant) can be used for Western blot and ELISA applications.

WARNING This product is not for human or veterinary use.

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