Host: E. coli • AA: 2-127 (full length) • MW: 14.04 kDa
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Citrullinated Histone H2B (human, recombinant)

Item No. 30133

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H2BC1
  • HIST1H2BA
  • hTSH2B
  • TH2B
  • TSH2B.1
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human histone H2B type 1-A expressed in E. coli, citrullinated with human recombinant PAD4
Amino Acids
2-127 (full length)
MW
14.04 kDa
Formulation (Request formulation change)
A solution in water
UniProt Accession №
Q96A08
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $602

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    Product Description

    Citrullinated Histone H2B Type 1-A is a citrullinated form of human histone H2B type 1-A (hTSH2B), a testis- and sperm-specific variant of the histone H2B protein that is encoded by H2BC1, previously known as HIST1H2BA.1,2 hTSH2B shares 85% identity with human somatic H2B.1, with the majority of amino acid differences between hTSH2B and somatic H2B occurring at the N-terminal end of the protein.1 H2BC1 is expressed in testis, and protein levels of hTSH2B are elevated in early round spermatids, decreasing over the course of spermiogenesis, with hTSH2B present in approximately 20% of mature spermatozoa.1,3 hTSH2B is enriched at promoter regions of genes important for sperm biology, capacitation, and fertilization and localizes to the basal nuclear area in mature spermatozoa.1,4 When incubated in Xenopus egg extracts, hTSH2B-positive human sperm cells decondense more rapidly and have increased long nuclear axis lengths compared with hTSH2B-negative sperm cells.5 Mice lacking the genes encoding the murine homolog of hTSH2B (TH2B), as well as the histone variant TH2A (Th2a-/-Th2b-/- mice), are sterile and have spermatogenesis defects.6 This product contains purified histone H2B type 1-A (human, recombinant) (Item No. 11081) that has been modified with PAD4 enzyme, which is subsequently depleted by affinity chromatography. Cayman's Citrullinated Histone H2B Type 1-A (human, recombinant) can be used for Western blot and ELISA applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zalensky, A.O., Siino, J.S., Gineitis, A.A., et al. Human testis/sperm-specific histone H2B (hTSH2B). Molecular cloning and characterization. The Journal of Biological Chemisty 277(45), 43474-43480 (2002).

    2. Marzluff, W.F., Gongidi, P., Woods, K.R., et al. The human and mouse replication-dependent histone genes. Genomics 80(5), 487-498 (2002).

    3. van Roijen, H.J., Ooms, M.P., Spaargaren, M.C., et al. Immunoexpression of testis-specific histone 2B in human spermatozoa and testis tissue. Hum. Reprod. 13(6), 1559-1566 (1998).

    4. Hammoud, S.S., Nix, D.A., Zhang, H., et al. Distinctive chromatin in human sperm packages genes for embryo development. Nature 460(7254), 473-478 (2009).

    5. Singleton, S., Mudrak, O., Morshedi, M., et al. Characterisation of a human sperm cell subpopulation marked by the presence of the TSH2B histone. Reprod. Fertil. Dev. 19(2), 392-397 (2007).

    6. Shinagawa, T., Huynh, L.M., Takagi, T., et al. Disruption of Th2a and Th2b genes causes defects in spermatogenesis. Development 142(7), 1287-1292 (2015).

    Product Citations

    Moon, J.-S., Younis, S., Ramadoss, N.S., et al. Cytotoxic CD8+ T cells target citrullinated antigens in rheumatoid arthritis. Nat. Commun. 14(1), 319 (2023).