For immunochemical detection of DNA
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DNA Monoclonal Antibody (Clone 4E9)

Item No. 30185

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • DNA Antibody
  • ss/dsDNA Monoclonal Antibody
Clone Designation
4E9
Formulation (Request formulation change)
100 µg of protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
IHC, IF, ELISA
Cross Reactivity
(+) Single- and double-stranded DNA, nuclei(-) Reduced protein lysate, histone
Species Reactivity
(+) Human DNA(+) Bovine DNA
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $440

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    NET Formation Screening & Analysis Services
    Our experts are here to help.
    • Detection of NET formation ex vivo and screening for modulators using:
      • High-content imaging
      • Enzymatic detection
      • Citrullination/carbamylation detection
    • Experienced scientists skilled in neutrophil biology, isolation, and handling
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    Product Description

    Activated neutrophils produce neutrophil extracellular traps (NETs), antimicrobial snares that are comprised of granular and cytoplasmic proteins, as well as decondensed nuclear DNA associated with histone proteins.1 NET degradation is impaired in patients with systemic lupus erythematosus (SLE), allowing for production of autoantibodies against NET components, including dsDNA. The NZBWF1 mouse model of SLE similarly produces autoantibodies against chromatin, dsDNA, and ssDNA.2,3 Cayman's DNA Monoclonal Antibody (Clone 4E9) was developed by fusing the spleen of a non-immunized NZBWF1 mouse with a mouse myeloma cell line and can be used for immunohistochemistry (IHC), immunofluorescence (IF), and ELISA applications. The antibody recognizes ssDNA and dsDNA, including nuclear DNA, from human and bovine samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yu, Y., and Su, K. Neutrophil extracellular traps and systemic lupus erythematosus. J. Clin. Cell. Immunol. 4(2), 139 (2013).

    2. van Bavel, C.C., Dieker, J.W., Tamboer, W.P., et al. Lupus-derived monoclonal autoantibodies against apoptotic chromatin recognize acetylated conformational epitopes. Mol. Immunol. 48(1-3), 245-256 (2010).

    3. Wu, W.-M., Lin, B.-F., Su, Y.-C., et al. Tamoxifen decreases renal inflammation and alleviates disease severity in autoimmune NZB/W F1 mice. Scand. J. Immunol. 52(4), 393-400 (2000).