In vitro Screening for Drug-Induced Hepatotoxicity using upcyte^® Hepatocytes Hepatotoxicity Assay

The principle application of this assay is to rapidly screen for test compounds to identify those that induce acute liver cell toxicity.

This kit includes two aliquots of cryopreserved Luminescent Reporter Hepatocytes (upcyte^®) prepared using INDIGO’s proprietary CryoMite^™ process. As with all cryopreserved mammalian cells, it is imperative that the aliquots of luminescent reporter hepatocytes are stored at temperatures no warmer than -78°C. When properly stored, the cryopreserved cells will yield high cell viability post-thaw (Figure 1) and provide the convenience of immediately dispensing cells into a 96-well assay plate. There is no need for intermediate handling steps such as spin-and-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup. An overnight culture period allows full recovery of the post-thaw hepatocytes and the establishment of a confluent cell monolayer that is ready to receive the user’s test compounds.

In addition to two aliquots of the reporter hepatocytes, the kit provides two cell culture-ready assay plates, optimized Cell Culture Medium-2 (CCM-2) for use in all steps of the assay procedure (cell thawing, seeding, and preparation of treatment media), luciferase detection reagent, and a reference compound that provides a positive control for hepatotoxicity.

The reagents and materials provided in this assay kit are formatted to allow the user to choose between two alternative assay setups. In one scenario 48 culture wells may be setup at two different times. In the other assay scenario 96 culture wells may be setup at one time.

[INDIGO Catalog No. ULC1003-48]

WARNING This product is not for human or veterinary use.