A colorimetric β-glucuronidase substrate
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Phenolphthalein β-D-Glucuronide (hydrate)

Item No. 30424

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-[1,3-dihydro-1-(4-hydroxyphenyl)-3-oxo-1-isobenzofuranyl]phenyl, β-D-glucopyranosiduronic acid, hydrate
Synonyms
  • Phenolphthalein β-Glucuronide
Molecular Formula
C26H22O10 • XH2O
Formula Weight
494.5
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 30 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
227 nm
SMILES
O[C@H]([C@H]1O)[C@H](O)[C@@H](C(O)=O)O[C@H]1OC(C=C2)=CC=C2C3(C4=CC=C(O)C=C4)OC(C5=CC=CC=C53)=O.OO[C@H]([C@H]1O)[C@H](O)[C@@H](C(O)=O)O[C@H]1OC(C=C2)=CC=C2C3(C4=CC=C(O)C=C4)OC(C5=CC=CC=C53)=O.O
InChi Code
InChI=1S/C26H22O10.H2O/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32;/h1-12,19-22,25,27-30H,(H,31,32);1H2/t19-,20-,21+,22-,25+,26?;/m0./s1
InChi Key
SZZQHDCPLMHIIA-PDFLYTMFSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 mg

    $36

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    10 mg

    $61

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    25 mg

    $95

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    Product Description

    Phenolphthalein β-D-glucuronide is a colorimetric substrate for β-glucuronidase.1 Upon enzymatic cleavage by β-glucuronidase, phenolphthalein is released, which can be quantified by colorimetric detection at 552 nm as a measure of β-galactosidase activity.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ball, A.L., Chambers, K.A., Hewinson, M., et al. A microtitre plate assay for measuring glycosidase activity. J. Enzyme Inhib. Med. Chem. 23(1), 131-135 (2008).

    2. Huang, Y.-L., and Chau, C.-F. Improvement in intestinal function of hamsters as influenced by consumption of polysaccharide-rich sage weed extracts. Food Chem. 133(4), 1618-1623 (2012).