A colorimetric reagent for the detection of copper
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2,2’-Biquinoline-4,4’-dicarboxylic Acid (sodium salt)

Item No. 30425

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
CAS Number
979-88-4
Synonyms
  • BCA
  • Bicinchoninic Acid
  • 2,2’-Bicinchoninic Acid
  • 4,4′-Dicarboxy-2,2′-biquinoline
Molecular Formula
C20H10N2O4 • 2Na
Formula Weight
388.3
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
PBS (pH 7.2): 10 mg/ml
λmax
264 nm
SMILES
O=C([O-])C1=CC(C2=CC(C([O-])=O)=C(C=CC=C3)C3=N2)=NC4=C1C=CC=C4.[Na+].[Na+]
InChi Code
InChI=1S/C20H12N2O4.2Na/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);;/q;2*+1/p-2
InChi Key
AUPXFICLXPLHBB-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu+).1,2 BCA and Cu+ form a stable 2:1 complex that has an absorbance maximum at 562 nm.1 It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu2+ is converted to Cu+ as a function of protein concentration under alkaline conditions.1,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Smith, P.K., Krohn, R.I., Hermanson, G.T., et al. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150(1), 76-85 (1985).

    2. Brenner, A.J., and Harris, E.D. A quantitative test for copper using bicinchoninic acid. Anal. Biochem. 226(1), 80-84 (1995).

    3. Walker, J.M. The bicinchoninic acid (BCA) assay for protein quantitation. Methods Mol. Biol. 32, 5-8 (1994).