A steroidal saponin with diverse biological activities
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Timosaponin BII

Item No. 30644

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(3β,5β,22α,25S)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurostan-3-yl 2-O-β-D-glucopyranosyl-β-D-galactopyranoside
CAS Number
136656-07-0
Synonyms
  • TB-II
Molecular Formula
C45H76O19
Formula Weight
921.1
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: slightly solublePBS (pH 7.2): 5 mg/ml
SMILES
C[C@H](CO[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)CC[C@@]2(O)O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC[C@]6([H])C[C@@H](O[C@H]7[C@H](O[C@H]8[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O8)[C@@H](O)[C@@H](O)[C@@H](CO)O7)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C
InChi Code
InChI=1S/C45H76O19/c1-19(18-58-40-37(55)34(52)31(49)27(15-46)60-40)7-12-45(57)20(2)30-26(64-45)14-25-23-6-5-21-13-22(8-10-43(21,3)24(23)9-11-44(25,30)4)59-42-39(36(54)33(51)29(17-48)62-42)63-41-38(56)35(53)32(50)28(16-47)61-41/h19-42,46-57H,5-18H2,1-4H3/t19-,20-,21+,22-,23+,24-,25-,26-,27+,28+,29+,30-,31+,32+,33-,34-,35-,36-,37+,38+,39+,40+,41-,42+,43-,44-,45+/m0/s1
InChi Key
SORUXVRKWOHYEO-UJDJZYEZSA-N
Origin
Plant/Anemarrhena asphodeloides
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $69

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    5 mg

    $280

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    10 mg

    $427

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    25 mg

    $901

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    Cayman Chemical
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    Product Description

    Timosaponin BII (TB-II) is a steroidal saponin that has been found in Anemarrhenae and has diverse biological activities.1,2,3 TB-II (20-100 µM) inhibits superoxide generation induced by phorbol 12-myristate 13-acetate (PMA; Item No. 10008014), N-formyl-Met-Leu-Phe (fMLP; Item No. 21495), or arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607) in isolated human neutrophils.1 It reduces cell death induced by enterovirus 71 (EV71) in Vero cells (IC50 = 4.3 µM).2 TB-II (20, 40, and 80 mg/ml) inhibits ADP-induced platelet aggregation in isolated rabbit platelet-rich plasma (PRP).3 TB-II (3 and 6 mg/kg) increases activated partial thromboplastin time (APTT) and decreases the wet weight and length of thrombi in Chandler’s ex vivo thrombosis model.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhang, J., Zhang, M., Sugahara, K., et al. Effect of steroidal saponins of Anemarrhenae rhizoma on superoxide generation in human neutrophils. Biochem. Biophys. Res. Commun. 259(3), 636-639 (1999).

    2. Liu, M., Tao, L., Chau, S.L., et al. Folding fan mode counter-current chromatography offers fast blind screening for drug discovery. Case study: Finding anti-enterovirus 71 agents from Anemarrhena asphodeloides. J. Chromatogr. A 1368, 116-124 (2014).

    3. Lu, W.-Q., Qiu, Y., Li, T.-J., et al. Antiplatelet and antithrombotic activities of timosaponin B‐II, an extract of Anemarrhena asphodeloides. Clin. Exp. Pharmacol. Physiol. 38(7), 430-434 (2011).