A fluorescent probe for lipid droplets
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Nile Red

Item No. 30787

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
9-(diethylamino)-5H-benzo[a]phenoxazin-5-one
CAS Number
7385-67-3
Molecular Formula
C20H18N2O2
Formula Weight
318.4
Purity
≥95%
Emission
576 nm, for neutral lipids
Excitation
529 nm, for neutral lipids
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlDMSO:PBS (pH 7.2) (1:10): 0.09 mg/mlEthanol: slightly soluble
λmax
265, 553 nm
SMILES
O=C1C=C2OC3=CC(N(CC)CC)=CC=C3N=C2C4=CC=CC=C14
InChi Code
InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChi Key
VOFUROIFQGPCGE-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $75

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    500 mg

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    1 g

    $222

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    Product Description

    Nile red is a fluorescent probe commonly used for the detection of intracellular lipid droplets.1 It displays excitation/emission maxima of 529/576 nm, respectively, for neutral lipids.2 It is selective for neutral lipids at emission wavelengths of 580 nm or lower, but, at higher emission wavelengths, phospholipid membranes are also visualized. Nile red has been used in live, fixed, or unfixed cells with detection by fluorescence microscopy, spectroscopy, or flow cytometry.1,3 It has been used as a marker of lipid droplets in cellular models of hepatocyte steatosis.4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Greenspan, P., Mayer, E.P., and Fowler, S.D. Nile red: A selective fluorescent stain for intracellular lipid droplets. J. Cell Biol. 100(3), 965-973 (1985).

    2. Greenspan, P., and Fowler, S.D. Spectrofluorometric studies of the lipid probe, nile red. J. Lipid. Res. 26(7), 781-789 (1985).

    3. Listenberger, L.L., and Brown, D.G. Fluorescent detection of lipid droplets and associated proteins. Curr. Protoc. Cell. Biol. 35(1), 24.22.21-24.22.11 (2007).

    4. Zheng, L., Lv, G.-C., Sheng, J., et al. Effect of miRNA-10b in regulating cellular steatosis level by targeting PPAR-ɑ expression, a novel mechanism for the pathogenesis of NAFLD. J. Gastroenterol. Hepatol. 25(1), 156-163 (2010).

    5. Rafiei, H., Omidian, K., and Bandy, B. Dietary polyphenols protect against oleic acid-induced steatosis in an in vitro model of NAFLD by modulating lipid metabolism and improving mitochondrial function. Nutrients 11(3), 541 (2019).