A fluorogenic substrate for SIRT1, SIRT2, and SIRT3
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Ac-QPKK(Ac)-AMC (trifluoroacetate salt)

Item No. 30790

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N2-acetyl-L-glutaminyl-L-prolyl-L-lysyl-N6-acetyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-lysinamide, trifluoroacetate salt
Synonyms
  • Ac-Gln-Pro-Lys-Lys-(Ac)-AMC
  • Ac-Gln-Pro-Lys-Lys-(Ac)-7-amino-4-methylcoumarin
  • p53317-320 Substrate (Ac-QPKK(Ac)-AMC)
Molecular Formula
C36H52N8O9 • XCF3COOH
Formula Weight
740.9
Purity
≥95%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 15 mg/mlEthanol: Slightly SolublePBS (pH 7.2): 10 mg/ml
SMILES
NCCCC[C@@H](C(N[C@H](C(NC1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)CCCCNC(C)=O)=O)NC([C@@H]3CCCN3C([C@H](CCC(N)=O)NC(C)=O)=O)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C36H52N8O9.C2HF3O2/c1-21-19-32(48)53-30-20-24(12-13-25(21)30)41-33(49)26(10-5-7-17-39-22(2)45)42-34(50)27(9-4-6-16-37)43-35(51)29-11-8-18-44(29)36(52)28(40-23(3)46)14-15-31(38)47;3-2(4,5)1(6)7/h12-13,19-20,26-29H,4-11,14-18,37H2,1-3H3,(H2,38,47)(H,39,45)(H,40,46)(H,41,49)(H,42,50)(H,43,51);(H,6,7)/t26-,27-,28-,29-;/m0./s1
InChi Key
RRDHKUNLSOMJIS-MROKUPSKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $81

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    5 mg

    $357

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    10 mg

    $633

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    25 mg

    $1,280

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    Product Description

    Ac-QPKK(Ac)-AMC is a fluorogenic substrate for sirtuin 1 (SIRT1), SIRT2, and SIRT3.1 It is selectively deacylated by SIRT1-3 over SIRT6. Upon enzymatic cleavage by SIRT1, SIRT2, or SIRT3, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify SIRT1, SIRT2, and SIRT3 activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Galleano, I., Schiedel, M., Jung, M., et al. A continuous, fluorogenic sirtuin 2 deacylase assay: Substrate screening and inhibitor evaluation. J. Med. Chem. 59(3), 1021-1031 (2017).