A fluorogenic substrate for trypsin, cathepsin B, and cathepsin H
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Z-(L-Arg)-AMC (hydrochloride)

Item No. 30797

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
[(1S)-4-[(aminoiminomethyl)amino]-1-[[(4-methyl-2-oxo-2H-1-benzopyran-7-yl)amino]carbonyl]butyl]-carbamic acid, phenylmethyl ester, monohydrochloride
CAS Number
70375-22-3
Synonyms
  • Bz-Arg-AMC
  • N-benzoxy-L-Arg-7-amido-4-methylcoumarin
  • N-benzoyl-L-Arg-AMC
  • Z-Arg(HCl)AMC
Molecular Formula
C24H27N5O5 • HCl
Formula Weight
502.0
Purity
≥98%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/ml
λmax
327 nm
SMILES
NC(NCCC[C@@H](C(NC1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)NC(OCC3=CC=CC=C3)=O)=N.Cl
InChi Code
InChI=1S/C24H27N5O5.ClH/c1-15-12-21(30)34-20-13-17(9-10-18(15)20)28-22(31)19(8-5-11-27-23(25)26)29-24(32)33-14-16-6-3-2-4-7-16;/h2-4,6-7,9-10,12-13,19H,5,8,11,14H2,1H3,(H,28,31)(H,29,32)(H4,25,26,27);1H/t19-;/m0./s1
InChi Key
JYWCRDUTYUBXOL-FYZYNONXSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $75

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    25 mg

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    50 mg

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    100 mg

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    Product Description

    Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2 Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zimmerman, M., Ashe, B., Yurewicz, E.C., et al. Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substrates. Anal. Biochem. 78(1), 47-51 (1977).

    2. Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al. Proteolytic degradation of host hemoglobin by schistosomes. Mol. Biochem. Parasitol. 89(1), 1-9 (1997).