A colorimetric lipase and esterase substrate
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4-Nitrophenyl Palmitate

Item No. 30870

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
hexadecanoic acid, 4-nitrophenyl ester
CAS Number
1492-30-4
Synonyms
  • p-Nitrophenyl Palmitate
  • para-Nitrophenyl Palmitate
  • pNpp
Molecular Formula
C22H35NO4
Formula Weight
377.5
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/ml
λmax
212, 270 nm
SMILES
O=C(CCCCCCCCCCCCCCC)OC1=CC=C([N+]([O-])=O)C=C1
InChi Code
InChI=1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3
InChi Key
LVZSQWIWCANHPF-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    1 g

    $36

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    5 g

    $128

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    10 g

    $236

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    25 g

    $462

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    Product Description

    4-Nitrophenyl palmitate is a colorimetric lipase and esterase substrate.1 Upon enzymatic hydrolysis of 4-nitrophenyl palmitate, 4-nitrophenol is released which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipases.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gupta, N., Rathi, P., and Gupta, R. Simplified para-nitrophenyl palmitate assay for lipases and esterases. Anal. Biochem. 311(1), 98-99 (2002).